- Clone
- P2C4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GJA1, Cx43, Gap Junction Protein Alpha 1
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![P2C4_PURE_Connexin_Antibody_1_WB_061417_resized P2C4_PURE_Connexin_Antibody_1_WB_061417_resized](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/P2C4_PURE_Connexin_Antibody_1_WB_061417_resized.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Western blot of purified anti-Connexin 43, 360-382 antibody (clone P2C4), and isotype-matched IgG2a, κ control. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of mouse brain lysate. Blots were incubated with 2 µg/ml of each primary antibody overnight at 4°C, followed by incubation with horseradish peroxidase labeled goat anti-mouse secondary antibody. Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Connexin 43, 360-382 antibody (clone P2C4) on formalin-fixed, paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with the primary antibody at 5 µg/ml overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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849601 | 25 µg | 95€ | ||||
849602 | 100 µg | 235€ |
Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated peptide corresponding to amino acids 360-382 of Connexin 43.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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WB - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody binds to the C-terminus of Connexin 43 and shows preference for non-phosphorylated Connexin 43 by western blotting.
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Application References
(PubMed link indicates BioLegend citation) -
- Chu G, et al. 2002. Cardiovasc. Res. 54(1):105. (WB)
- Wei CJ, et al. 2005. J. Biol. Chem. 280(20):19925. (WB)
- RRID
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AB_2687296 (BioLegend Cat. No. 849601)
AB_2687297 (BioLegend Cat. No. 849602)
Antigen Details
- Structure
- Connexin 43 (GJA1) is a 382 amino acid protein with a molecular mass of 43 kD.
- Distribution
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Tissue distribution: Expressed in most tissues, enriched in brain, endocrine, muscle, bone marrow, immune System, ovaries, testes, and Skin.
Cellular distribution: Plasma membrane, cytosol, mitochondria, golgi apparatus, cytoskeleton and endoplasmic reticulum membrane. - Function
- Connexin 43 plays a role in cell-to-cell communication by forming channels, or gap junctions between cells. It allows for the transport of nutrients, charged particles (ions), and other small molecules that carry necessary communication signals between cells.
- Molecular Family
- Gap Junction Proteins
- Antigen References
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1. Grek CL, et al. 2016. Cancer Lett. 374(1):117.
2. Lilly E, et al. 2016. Semin. Cell Dev. Biol. Feb; 50:4
3. Michela P, et al. 2015. Eur. J. Pharmacol. 768:71-6. - Gene ID
- 2697 View all products for this Gene ID
- UniProt
- View information about Connexin 43 360-382 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Connexin 43, 360-382 Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-Connexin 43, 360-382 | P2C4 | WB,IHC-P |
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Purified anti-Connexin 43, 360-382
Western blot of purified anti-Connexin 43, 360-382 antibody ... IHC staining of purified anti-Connexin 43, 360-382 antibody ...
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