Purified anti-Cytokeratin Antibody

Pricing & Availability
Clone
AE-1 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AE-1_PURE_Cytokeratin_Antibody_1_082219
IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-1_PURE_Cytokeratin_Antibody_1_082219
    IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-1_PURE_Cytokeratin_Antibody_2_082219
    IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded dog skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • AE-1_PURE_Cytokeratin_Antibody_3_082219
    IHC staining of purified anti-Cytokeratin antibody (clone AE-1) on formalin-fixed paraffin-embedded primate skin tissue. Following antigen retrieval using Retrieve-All Antigen Unmasking System 1: Universal, 100X (Cat. No. 927901), the tissue was incubated with 0.5 µg/ml of the primary antibody for 20 minutes at room temperature. A biotinylated anti-mouse linking reagent followed by alkaline phosphatase conjugated streptavidin and fast red chromogen was used for detection. Hematoxylin was used for counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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914004 100 µg 184€
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Description

This antibody recognizes low and intermediate molecular weight type I acidic keratins (40, 48, 50, and 56.5 kD). This clone stains the basal layer of epidermis and most epithelia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Yang K, et al. 1990. Amer. J. of Clin. Path. 94:261. (IHC) PubMed
RRID
AB_2616958 (BioLegend Cat. No. 914004)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Cytokeratin on UniProt.org
Go To Top Version: 3    Revision Date: 08.16.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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