- Clone
- AFL4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vascular endothelial growth factor receptor 3, FLT-4, Fms-like tyrosine kinase 4
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![AFL4_Pure_0_061919 AFL4_Pure_0_061919](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/AFL4_Pure_0_061919.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total cell lysates (15 µg total protein) from HEK293T cells mock-transfected or transfected with a plasmid containing FLT4 were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-VEGFR-3 (FLT-4) Antibody, clone AFL4, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG Antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. -
6 µm frozen thymus section from a naïve C57BL/6 mouse was stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green) and DAPI (blue). Magnification 40X. -
6 µm frozen lung section from an influenza-infected C57BL/6 mouse stained with purified anti-VEGFR-3 (clone AFL4), followed by incubation with goat anti-rat IgG Alexa Fluor® 488 (green), anti-CD3 (red) and DAPI (blue). Magnification 40X.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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140901 | 50 µg | 67€ | ||||
140902 | 500 µg | 207€ |
VEGFR-3, also known as FLT4, is a member of the Tyr protein kinase family. The extracellular portion of VEGFR-3 contains 7 immunoglobulin (Ig)-like domains and the cytoplasmic portion contains a protein kinase domain. FLT4 regulates angiogenesis and lymphangiogenesis, its ligands are VEGF-C and D and its binding is mediated by the 2nd and 3rd Ig-like domains of FLT4. During fetal development VEGFR-3 is expressed on endothelial cells, however, in the adult mice, the vascular endothelial cells lose VEGFR-3 expression, but the lymphatic endothelium expresses it constitutively. In addition, VEGFR-3 expression can be induced in tumors with active angiogenesis.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- VEGFR-3-Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
Block, ELISA, IP, IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: ELISA1,2, immunoprecipitation1and Western blot1,2 from cell lysates, immunohistochemical staining1, 3 of frozen and paraffin embedded tissue sections and in whole-mount mouse embryos, in vivo blocking1, 5 of VEGF-C binding to inhibit angiogenesis, lymphangiogenesis, growth and metastasis of different tumor cells, in vitro blocking4 of VEGF-C binding to induce dispersion of endothelial cell clusters.
This clone detect multiple bands by western blot: a ~140 kD band corresponding to cleaved/soluble VEGFR-3, and 220 and 230 kD bands corresponding to unglycosylated and glycosylated full length VEGFR-3 (Bando, et al. 2004. Int J Canc. 111:184.) - Additional Product Notes
-
This product has been reported by a collaborator to work for immunohistochemical staining of frozen tissue sections.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kubo H, et al. 2000. Blood 96:546. (IP, WB, IHC, ELISA, Block)
- Zhang L, et al. 2010. Cell. Res. Epub ahead of print (WB, ELISA)
- Tammela T, et al. 2010. Nature 454:656. (IHC)
- Shimizu K, et al. 2004. Cancer Sci. 95:328. (Block)
- Matsumura K, et al. 2003. Blood 101:1367. (Block)
- Lee J, et al. 2012. PNAS 109:19638. PubMed
- Ozasa R, et al. 2012. J Exp Clin Cancer Res. 31:83. PubMed
- Product Citations
-
- RRID
-
AB_10683463 (BioLegend Cat. No. 140901)
AB_10680790 (BioLegend Cat. No. 140902)
Antigen Details
- Structure
- Member of Tyr protein kinase family. Extracellular portion contains 7 immunoglobulin-like domains. Ligand binding is mediated by its domains 2 and 3. Cytoplasmic portion contains 1 protein kinase domain.
- Distribution
-
Expressed on vascular endothelial cells during fetal development, constitutively expressed on lymphatic endothelium in adult mice, induced in tumors with active angiogenesis.
- Function
- Regulates angiogenesis and lymphangiogenesis.
- Interaction
- Associated with VEGFR-2 in the surface.
- Ligand/Receptor
- VEGF-C and D
- Cell Type
- Endothelial cells
- Biology Area
- Angiogenesis, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Cytokine/Chemokine Receptors, Protein Kinases/Phosphatase
- Antigen References
-
1. Uhrin P, et al. 2010. Blood 115:3997.
2. Wang Y, et al. 2010. Nature 465:483.
3. Akobsson L, et al. 2009. Biochem. Soc. T. 37:1233.
4. Tammela T, et al. 2010. Nature 454:656.
5. Huang G and Chen L. 2008. Cancer Biother. Radio. 23:661.
6. Zhang YJ and Fang JY. 2008. J. Gastroen. Hepatol. 23:856. - Gene ID
- 14257 View all products for this Gene ID
- UniProt
- View information about VEGFR-3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All VEGFR-3 (FLT-4) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse VEGFR-3 (FLT-4) | AFL4 | WB,IHC-F,Block,ELISA,IP |
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