- Clone
- 1H4B01 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TIR domain-containing adapter molecule 1, TRIF, MyD88-3, Proline-Rich, Vinculin And TIR Domain-Containing Protein B (PRVTIRB)
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_1H4B01_PURE_TICAM1_Antibody_WB_113017 A_1H4B01_PURE_TICAM1_Antibody_WB_113017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1H4B01_PURE_TICAM1_Antibody_WB_113017.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Total lysates (15 µg protein) from HepG2 (lane 1) and HeLa (lane 2) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:500 diluted (1 µg/mL) purified anti-TICAM-1 antibody (clone 1H4B01, upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted HRP goat anti-mouse IgG secondary antibody for the anti-TICAM-1 antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1, lower). Lane M: Molecular weight ladder. -
Raji cells were stained with purified anti-TICAM-1 (clone 1H4B01) antibodies, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green) antibody. Nuclei were stained with DAPI (blue). -
Immunoprecipitation of TICAM1 from Raji cell extracts. Lane 1 is 5% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-TICAM1 antibody (clone 1H4B01, lane 4). Western blot was performed using anti-TICAM1 antibody (clone 1H4B01). -
IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B01) on formalin-fixed paraffin-embedded human small intestine tissue. Following antigen retrieval using 1X Tris-EDTA, Ph 9.0, the tissue was incubated with (B) or without (A) 1.0 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM -
IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B01) on formalin-fixed paraffin-embedded human cortex tissue. Following antigen retrieval using 1X Tris-EDTA, pH 9.0, the tissue was incubated with (B) or without (A) 10.0 μg/mL of antibody overnight at 4°C, followed by incubation with 2.5 μg/mL of Alexa Fluor® 647 goat anti-mouse IgG (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scalebar = 50 μM
Cat # | Size | Price | Quantity Check Availability | Save | ||
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657102 | 100 µg | 212€ |
TICAM-1, also known as TRIF, is an intracellular adaptor protein utilized by TLR3 and TLR4. TLR3 recognizes double stranded viral RNA and induces TICAM-1 mediated type I interferon production. The N-terminal region of TICAM-1 recruits TBK1 and IKKi, which in turn phosphorylates the interferon regulatory transcription factor IRF3. The phosphorylated IRF3 is subsequently dimerized and translocates to the nucleus, resulting in transcriptional activation of the gene encoding IFN-β. Mice lacking in TICAM-1 expression are defective in the TLR3 mediated induction of proinflamatory cytokines, suggesting that TICAM-1 is crucial for TLR3 downstream signaling. TICAM-1 also mediates TLR4-induced signaling in the presence of the adaptor molecule TRAM. The C-terminal region of TICAM-1 recruits RIP1 and induces apoptosis through FADD/caspase cascade.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human TICAM-1 recombinant protein (29-204 a.a.)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IP, IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.0 - 10 μg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
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- RRID
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AB_2562543 (BioLegend Cat. No. 657102)
Antigen Details
- Structure
- 712 amino acids, predicted molecular weight of 76 kD. Contains a Toll/interleukin-1 receptor (TIR) homology domain responsible for mediating protein-protein interactions.
- Distribution
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Cytosol, endosome membrane
- Function
- TICAM-1 acts as an adaptor protein of TLR3 and TLR4 (through TRAM), mediating innate immune responses during viral infection.
- Interaction
- TICAM-1 interacts with TLR3, TRAM, TBK1, IRF3, IRF7, TRAF3, TRAF6, and IKKi.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Toll Like Receptors
- Antigen References
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1. Petnicki-Ocwieja T, et al. 2013. Infect Immun. 81:402.
2. Riad A, et al. 2011. J. Immunol. 186:2561.
3. Choi YJ, et al. 2010. J. Biol. Chem. 285:21382.
4. Gais P, et al. 2010. J. Immunol. 184:5842.
5. Han KJ, et al. 2010. J. Biol. Chem. 285:12543.
6. Takeda K and Akira S. 2005. Int. Immunol. 17:1. - Gene ID
- 148022 View all products for this Gene ID
- UniProt
- View information about TICAM-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TICAM-1 (TRIF) Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-TICAM-1 (TRIF) | 1H4B01 | WB,ICC,IP,IHC-P |
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Purified anti-TICAM-1 (TRIF)
Total lysates (15 µg protein) from HepG2 (lane 1) and HeLa (... Raji cells were stained with purified anti-TICAM-1 (clone 1H... Immunoprecipitation of TICAM1 from Raji cell extracts. Lane ... IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B0... IHC staining using purified anti-TICAM-1 (TRIF) (clone 1H4B0...
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