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Alexa Fluor® 488 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
1_UCHT1_AF488_071105
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 488®
  • 1_UCHT1_AF488_071105
    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor 488®
  • 2_UCHT1_A488_CD3_Antibody_ICC_012121
    Human peripheral blood mononuclear cells and neutrophil mixed cells were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD11b (clone ICRF44) Alexa Fluor® 594 (red) and 5 µg/ml CD3 (clone UCHT1) Alexa Fluor® 488 (green) for 30 minutes at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured by 40X objective.
  • 3_UCHT1_A488_CD3_Antibody_IHC-F_012121
    Human frozen tonsil tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 5 µg/mL of Alexa Fluor® 488 anti-human CD3 antibody (green) and Brilliant Violet 421™ anti-human CD19 antibody (blue) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
300454 100 µg 198€
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300415 100 tests 215€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC, IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume For flow cytometric staining using the test size, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Marquardsen F, et al. 2017. J Clin Immunol. 10.1007/s10875-017-0431-5. PubMed
  2. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  3. Burel JG, et al. 2019. Elife. 8. PubMed
  4. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  5. Chen M, et al. 2019. Cell Stem Cell. 25:501. PubMed
  6. VanSaun M, et al. 2013. PLoS One. 8:73054. PubMed
  7. Kerstein A, et al. 2016. J Autoimmun. S0896-8411(16)30186-X. PubMed
  8. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  9. Wallace JG, et al. 2021. J Allergy Clin Immunol. 147:743. PubMed
  10. Keck S, et al. 2021. Cellular and Molecular Gastroenterology and Hepatology. 12(2):507-545. PubMed
  11. Özcan A, et al. 2020. J Invest Dermatol. 1003:140. PubMed
  12. Tomita T, et al. 2021. Nat Commun. 12:3655. PubMed
  13. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  14. Khosravi-Maharlooei M, et al. 2021. J Autoimmun. 119:102612. PubMed
  15. Li C, et al. 2022. J Exp Med. 219:. PubMed
  16. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  17. Riding AM, et al. 2022. iScience. 25:104660. PubMed
  18. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  19. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  20. Riese P, et al. 2022. Nat Commun. 13:6894. PubMed
  21. Gilman KE, et al. 2023. Front Immunol. 13:1045710. PubMed
  22. Tolcher AW, et al. 2023. J Immunother Cancer. 11: . PubMed
  23. Hu X, et al. 2023. Nat Commun. 14:2020. PubMed
  24. Hu X, et al. 2023. Nat Biotechnol. . PubMed
RRID
AB_2564149 (BioLegend Cat. No. 300454)
AB_389310 (BioLegend Cat. No. 300415)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 4    Revision Date: 01.21.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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