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Alexa Fluor® 488 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Kathleen-Ignatoski.jpg
Frozen mouse intestine stained with purified anti-Ankyrin-B (green), E-Cadherin (red) and DAPI (blue) followed by anti-goat Alexa Fluor® 488 and anti-rabbit Alexa Fluor® 568. Image generously submitted to the 2017 Cell Life Imaging Competition by Kathleen Ignatoski from University of Michigan.
  • Kathleen-Ignatoski.jpg
    Frozen mouse intestine stained with purified anti-Ankyrin-B (green), E-Cadherin (red) and DAPI (blue) followed by anti-goat Alexa Fluor® 488 and anti-rabbit Alexa Fluor® 568. Image generously submitted to the 2017 Cell Life Imaging Competition by Kathleen Ignatoski from University of Michigan.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Check Availability Save
405319 100 µg 147€
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Tomić S, et al. 2017. Biomaterials. 10.1016/j.biomaterials.2017.08.040. PubMed
  2. Bidet K, et al. 2019. NPJ Vaccines. 4:27. PubMed
  3. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
  4. Alberts A, et al. 2020. Sci Rep. 10:6391. PubMed
  5. Trapecar M, et al. 2020. Cell Systems. 10(3):223-239. PubMed
  6. Brown CW, et al. 2020. Developmental Cell. 51(5):575-586.e4.. PubMed
  7. Wu H, et al. 2016. Sci Rep. 6:28065. PubMed
  8. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  9. Dhande IS, et al. 2020. J Am Heart Assoc. 9:e014142. PubMed
  10. Trapecar M, et al. 2021. Sci Adv. 7:00. PubMed
  11. Wang X, et al. 2020. Immunity. 328:52. PubMed
  12. Slabicki M, et al. 2020. Nature. 588:164. PubMed
  13. Rahimipour M, et al. 2021. Cell J. 23:154. PubMed
  14. Duran J, et al. 2020. Mol Neurobiol. 57:4657. PubMed
  15. Nie Y, et al. 2021. Virol Sin. 36:692. PubMed
  16. Bianchi E, et al. 2021. Nat Commun. 12:1251. PubMed
  17. Fu W, et al. 2021. Front Immunol. 12:656090. PubMed
  18. Varea O, et al. 2021. Neurobiol Dis. 147:105173. PubMed
  19. Kim J, et al. 2022. Sci Rep. 12:7753. PubMed
  20. Zelenova EA, et al. 2021. Cells. 10:. PubMed
  21. Varea O, et al. 2022. Brain Commun. 4:fcac168. PubMed
  22. Liu Y, et al. 2022. J Inflamm Res. 15:4315. PubMed
  23. Chen BR, et al. 2022. Methods Mol Biol. 2444:15. PubMed
  24. Wennerberg E, et al. 2022. Sci Transl Med. 14:eabe8195. PubMed
  25. Moore ER, et al. 2021. Bone. 143:115738. PubMed
  26. Chandler A, et al. 2022. J Orthop Res. 40:1918. PubMed
RRID
AB_2563044 (BioLegend Cat. No. 405319)

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Go To Top Version: 4    Revision Date: 01.27.2021

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