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Alexa Fluor® 700 anti-human CD45RA Antibody

Pricing & Availability
Clone
HI100 (See other available formats)
Regulatory Status
RUO
Workshop
IV N906
Other Names
GP180, L-CA, LCA, LY5, T200, PTPRC
Isotype
Mouse IgG2b, κ
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Product Citations
publications
HI100_Alx700_043009.jpg
Human peripheral blood lymphocytes stained with HI100 Alexa Fluor® 700
  • HI100_Alx700_043009.jpg
    Human peripheral blood lymphocytes stained with HI100 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Check Availability Save
304119 25 µg 100€
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304120 100 µg 212€
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Description

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: inhibition of CD45 functions2, immunohistochemical staining of frozen tissue sections3 and formalin-fixed paraffin-embedded tissue sections4, and immunocytochemistry15,16.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Yamada T, et al. 2002. J. Biol. Chem. 277:28830. (WB, Block)
  3. Weninger W, et al. 2003 J. Immunol. 170:4638. (IHC-F)
  4. Imanguli MM, et al. 2009. Blood. 113:3620 (IHC-P)
  5. Roque S, et al. 2007. J. Immunol. 178:8028. (FC) PubMed
  6. Smeltz RB. 2007. J. Immunol. 178:4786. (FC) PubMed
  7. Palendira U, et al. 2008. Blood (FC) PubMed
  8. Kuttruff S, et al. 2009. Blood 113:358. (FC) PubMed
  9. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  10. Alanio C, et al. 2010. Blood 115:3718. (FC) PubMed
  11. Iannello A, et al. 2010. J. Immunol. 184:114. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Guereau-de-Arellan M, et al. 2011. Brain. 134:3578. PubMed
  14. Canque B, et al. 2000. Blood 96:3748. (ICC)
  15. Imanguli MM, et al. 2009. Blood 13:3620. (ICC)
  16. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Powell R, et al. 2017. J Immunol. 10.4049/jimmunol.1700114. PubMed
  2. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  3. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  4. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
  5. Good Z, et al. 2019. Nat Biotechnol. 37:259. PubMed
  6. Evans RDR, et al. 2020. Nat Commun. 3.491666667. PubMed
  7. Palendira U, et al. 2008. Blood. 112:3293. PubMed
  8. Payne R, et al. 2010. J Virol. 84:10453. PubMed
  9. Alanio C, et al. 2010. Blood. 115:3718. PubMed
  10. Carney E, et al. 2012. J Immunol. 189:261. PubMed
  11. Alexander T, et al. 2013. Ann Rheum Dis. 72:1549. PubMed
  12. Eriksson E, et al. 2012. PLoS One. 7:e51696. PubMed
  13. Long H, et al. 2013. J Exp Med. 210:933. PubMed
  14. Afzali B, et al. 2013. Clin J Am Soc Nephrol. 8:1396. PubMed
  15. Eberhardt K, et al. 2015. Clin Infect Dis. 61: 1615 - 1623. PubMed
  16. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  17. Muenchhoff M, et al. 2016. Sci Transl Med. 8: 358ra125. PubMed
  18. Kim Y, et al. 2021. Oncol Lett. 1.022222222. PubMed
  19. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  20. Pais Ferreira D, et al. 2020. Immunity. 53(5):985-1000.e11. PubMed
  21. Goc J, et al. 2021. Cell. 184:5015. PubMed
  22. Leclercq G, et al. 2021. J Immunother Cancer. 9: . PubMed
  23. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  24. Karim F, et al. 2021. Elife. 10:. PubMed
  25. Poon MML, et al. 2021. Cell Rep. 37:110071. PubMed
  26. Leclercq G, et al. 2022. J Immunother Cancer. 10:. PubMed
  27. Suwandi JS, et al. 2020. J Autoimmun. 107:102361. PubMed
  28. Baskar R, et al. 2022. Cell Rep Methods. 2:. PubMed
  29. Touizer E, et al. 2023. iScience. 26:105862. PubMed
  30. Fischer JR, et al. 2023. Cell Rep Med. 4:100977. PubMed
  31. Koutsakos M, et al. 2023. Immunity. 56:879. PubMed
  32. Yan F, et al. 2023. Front Cell Infect Microbiol. 13:1152987. PubMed
  33. Doloff JC, et al. 2023. Sci Adv. 9:eade9488. PubMed
RRID
AB_493762 (BioLegend Cat. No. 304119)
AB_493763 (BioLegend Cat. No. 304120)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane (exon 4 splicing of CD45 gene), 205-220 kD
Distribution

B cells, naïve T cells, monocytes

Function
Enhances TCR and BCR signaling
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45RA on UniProt.org
Go To Top Version: 3    Revision Date: 06.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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