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APC anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2
Human peripheral blood monocytes stained with M5E2 APC
  • M5E2
    Human peripheral blood monocytes stained with M5E2 APC
See APC spectral data
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301807 25 tests 52€
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301808 100 tests 108€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Kiers D et al. 2018. EBioMedicine. 33:144-156 . PubMed
  2. Powell EJ, et al. 2018. J Dairy Sci. 101:8182. PubMed
  3. Zhou Z, et al. 2018. J Dairy Sci. 101:10374. PubMed
  4. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  5. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  6. Broeren MGA, et al. 2018. ALTEX. 36:18:00. PubMed
  7. Alharthi AS, et al. 2019. J Dairy Sci. 102:10599. PubMed
  8. Kedage V, et al. 2019. MAbs. 12:1685832. PubMed
  9. Ferguson Bennit HR, et al. 2017. Blood Lymphat Cancer. 7:9. PubMed
  10. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  11. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  12. Mahmud FJ, et al. 2020. J Neuroinflammation. 0.897916667. PubMed
  13. Spildrejorde M, et al. 2014. Physiol Genomics. 46:512. PubMed
  14. Offersen R, et al. 2016. J Virol. 90: 4441 - 4453. PubMed
  15. Wang H, et al. 2017. J Neurochem. 141:347. PubMed
  16. Miksiunas R, et al. 2020. Int J Mol Sci. 21:00. PubMed
  17. Constantinescu-Bercu A, et al. 2020. eLife. 9:00. PubMed
  18. Bade RM, et al. 2021. Molecular Oncology. . PubMed
  19. da Rocha Sobrinho HM, et al. 2021. Journal of Neuroscience Research. 99:2511. PubMed
  20. Jankeel A, et al. 2020. J Virol. :94. PubMed
  21. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  22. Wegner J, et al. 2021. Sci Rep. 11:14983. PubMed
  23. Lopes MG, et al. 2021. J Dairy Sci. 104:9340. PubMed
  24. Downes DJ, et al. 2021. Nat Genet. 53:1606. PubMed
  25. Magg T, et al. 2021. Sci Immunol. 6:. PubMed
  26. Chen Y, et al. 2021. Front Mol Biosci. 8:777370. PubMed
  27. Thomas BL, et al. 2022. J Dairy Sci. 105:2256. PubMed
  28. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  29. Guo Z, et al. 2022. NPJ Precis Oncol. 6:34. PubMed
  30. Duncan CJA, et al. 2022. J Exp Med. 219:. PubMed
  31. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  32. Ferrari M, et al. 2021. J Virol. 95:e0068521. PubMed
  33. Claus C, et al. 2021. Pharmaceutics. 13:. PubMed
  34. Weiss R, et al. 2021. Cells. 10:. PubMed
  35. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  36. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  37. Takele Y, et al. 2022. PLoS Negl Trop Dis. 16:e0010544. PubMed
  38. Yokoyama K, et al. 2022. Front Immunol. 13:1016914. PubMed
  39. Vondra S, et al. 2023. Cell Rep. 42:111977. PubMed
  40. Fehlberg LK, et al. 2023. J Dairy Sci. :. PubMed
  41. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
RRID
AB_314189 (BioLegend Cat. No. 301807)
AB_314190 (BioLegend Cat. No. 301808)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 4    Revision Date: 11.05.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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