- Clone
- 15-414 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- BIT, MFR, P84, MYD-1, SHPS1, PTPNS1, CD172 antigen-like family member A
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![15-414_APC_CD172a_Antibody_022017 15-414_APC_CD172a_Antibody_022017](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/15-414_APC_CD172a_Antibody_022017.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood monocytes were stained with CD172a (clone 15-414) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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372105 | 25 tests | 110€ | ||||
372106 | 100 tests | 237€ |
CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KG1a cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Majdic O, et al. 1981. Blood. 58:1127.
- Product Citations
- RRID
-
AB_2650862 (BioLegend Cat. No. 372105)
AB_2650863 (BioLegend Cat. No. 372106)
Antigen Details
- Structure
- Type I transmembrane protein, 85-90 kD, one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, two ITIM motifs and a proline-rich region in the cytoplasmic tail.
- Distribution
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Monocytes, macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells.
- Function
- Negative regulation of receptor tyrosine kinase-coupled signaling, mediates macrophage multinucleation, involved in neuronal survival.
- Ligand/Receptor
- CD47, SP-A, and SP-D.
- Cell Type
- Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
- Biology Area
- Cell Biology, Immunology, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
1. Lv Z, et al. 2015. J. Immunol. 195:661.
2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024.
3. Zen K, et al. 2013. Nat. Commun. 4:2436.
4. Baba N, et al. 2013. J. Exp. Med. 210:1251.
5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457. - Gene ID
- 140885 View all products for this Gene ID
- UniProt
- View information about CD172alpha on UniProt.org
Related Pages & Pathways
Pages
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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