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APC anti-human CD29 Antibody

Pricing & Availability
Clone
TS2/16 (See other available formats)
Regulatory Status
RUO
Workshop
V A-S202
Other Names
Integrin β1 chain, VLA-β chain, gpIIa, ITGB1
Isotype
Mouse IgG1, κ
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Product Citations
publications
TS216_APC_062107
Human peripheral blood lymphocytes stained with TS2/16 APC
  • TS216_APC_062107
    Human peripheral blood lymphocytes stained with TS2/16 APC
See APC spectral data
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303007 25 tests 120€
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303008 100 tests 252€
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Description

CD29 is a 130 kD single chain type I glycoprotein also known as integrin β1, VLA-β chain, or gpIIa. It is broadly expressed on a majority of hematopoietic and non-hematopoietic cells, including leukocytes (although at low level on granulocytes), platelets, fibroblasts, endothelial cells, epithelial cells, and mast cells. CD29 is a member of the integrin family. It is non-covalently associated with integrin α1-α6 chains to form VLA-1 to VLA-6 molecules, respectively. Integrins, which include CD29, bind to several cell surface (e.g. VCAM-1, MadCAM-1) and extracellular matrix molecules. CD29 acts as a fibronectin receptor and is involved in a variety of cell-cell and cell-matrix interactions.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen tissue sections3,5, and activation of integrin ß14,7,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303010). Clone TS2/16 recognizes epitope A2.10

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Gutierrez-Lopez M, et al. 2003. J. Biol. Chem. 278:208.
  3. Hemler ME, et al. 1984. J. Immunol. 132:3011. (IHC, IP)
  4. Sanchez-Aparicio P, et al. 1994. J. Cell Biol. 126:271. (Activ)
  5. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC)
  6. Murga M, et al. 2005. Blood 105:1992. (FC) PubMed
  7. Porter JC and Hogg N. 1997. J. Cell Biol. 138:1437. (Activ)
  8. Conway RE, et al. 2006. Mol. Cell. Biol. 26:5310. (Activ)
  9. Wesseling J, et al. 1995. J. Cell. Biol. 129:255. (Dog Reactivity)
  10. Rubio G, et al. 2002. Cancer Immunol. Immunother. 51:130.
  11. Dong A, et al. 2015. J Biol Chem. 290:8016. PubMed
  12. Paebst F, et al. 2014. Cytometry A. 85(8):678-87. (Horse reactivity)
Product Citations
  1. Ding S, et al. 2018. Sci Rep. 8:10808. PubMed
  2. Judson RN et al. 2018. Cell stem cell. 22(2):177-190 . PubMed
  3. Yang C et al. 2019. Int J Mol Med. 43(3):1395-1405 . PubMed
  4. Wang T, et al. 2019. Mol Med Rep. 19:4719. PubMed
  5. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
  6. Frazier T, et al. 2018. Adipocyte. 0.41875. PubMed
  7. Sarkar TJ, et al. 2020. Nat Commun. 11:1545. PubMed
  8. Liu B, et al. 2017. Hum Cell. 30:51. PubMed
  9. Mancuso RV, et al. 2020. British Journal of Pharmacology. 177(12):2696-2711.. PubMed
  10. Murga M, et al. 2005. Blood. 105:1992. PubMed
  11. Huijskens M, et al. 2014. J Leukoc Biol. 96:1165. PubMed
  12. Chen G, et al. 2015. J Endod. 41: 1091-1099. PubMed
  13. Vishnoi M, et al. 2015. Sci Rep. 5: 17533. PubMed
  14. Quarta M, et al. 2016. Nat Biotechnol. 10.1038/nbt.3576. PubMed
  15. Zhou X, et al. 2020. Cells. 9(4):. PubMed
  16. Chulpanova DS, et al. 2020. Bioengineering (Basel). 7:00. PubMed
  17. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  18. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  19. Guney-Esken G, et al. 2021. Stem Cell Res Ther. 12:287. PubMed
  20. Tabebordbar M, et al. 2021. Cell. 184:4919. PubMed
  21. Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed
  22. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  23. Hegewisch-Solloa E, et al. 2021. J Immunol. 207:950. PubMed
  24. Kang DH, et al. 2021. Nat Commun. 12:5059. PubMed
  25. Lu H, et al. 2020. Am J Reprod Immunol. 83:. PubMed
  26. Liao HQ, et al. 2021. Am J Reprod Immunol. 86:e13406. PubMed
  27. Guney-Esken G, et al. 2021. Methods Mol Biol. 2549:23. PubMed
  28. Kitaeva KV, et al. 2022. Bioengineering (Basel). 9:. PubMed
  29. Benjamin DI, et al. 2022. Cell Metab. 34:902. PubMed
  30. Park S, et al. 2022. Biomolecules. 12: . PubMed
  31. McCurdy S, et al. 2022. FASEB J. 36:e22629. PubMed
  32. Gilazieva Z, et al. 2022. Curr Issues Mol Biol. 44:5363. PubMed
  33. Wei X, et al. 2022. Mol Cell. 82:1225. PubMed
  34. He Y, et al. 2023. Protein Cell. 14:202. PubMed
  35. Yen FC, et al. 2023. Nat Commun. 14:2942. PubMed
RRID
AB_314323 (BioLegend Cat. No. 303007)
AB_314324 (BioLegend Cat. No. 303008)

Antigen Details

Structure
Integrin, type I glycoprotein, forms VLA-1 to VLA-6 heterodimers with CD49a-f (α1-α6), also associates with CD51 (αV), and α7- α9, 130 kD
Distribution

Lymphocytes, monocytes, granulocytes (low), platelets, mast cells, fibroblasts, endothelial cells

Function
Cell-cell and cell-matrix interactions
Ligand/Receptor
VCAM-1, MAdCAM-1, ECM
Cell Type
Embryonic Stem Cells, Endothelial cells, Fibroblasts, Granulocytes, Lymphocytes, Mast cells, Mesenchymal Stem Cells, Monocytes, Platelets, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Hemler M. 1990. Annu. Rev. Immunol. 8:365.
2. Hynes R. 1992. Cell 69:11.

Gene ID
3688 View all products for this Gene ID
UniProt
View information about CD29 on UniProt.org
Go To Top Version: 4    Revision Date: 01.26.2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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