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APC anti-human CD45 Antibody

Pricing & Availability
Clone
HI30 (See other available formats)
Regulatory Status
RUO
Workshop
IV N816
Other Names
LCA, T200
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI30_APC_071307
Human peripheral blood lymphocytes stained with HI30 APC
  • HI30_APC_071307
    Human peripheral blood lymphocytes stained with HI30 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
304011 25 tests 36€
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304012 100 tests 84€
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304037 500 tests 269€
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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD45 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections9, inhibition of CD45 functions4, immunofluorescence11, Western blotting3, and spatial biology (IBEX)16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Kishihara K, et al. 1993. Cell 74:143.
  3. Esser M, et al. 2001. J. Virol. 75:6173. (WB)
  4. Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
  5. Nagano M, et al. 2007. Blood 110:151.
  6. Jiang Q, et al. 2008. Blood 112:2858. PubMed
  7. Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  10. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  11. Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
  12. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  13. Breton G, et al. 2015. J Exp Med. 212:401. PubMed
  14. Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
  15. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Park E, et al. 2017. Methods Mol Biol. 10.1007/978-1-4939-7144-2_2. PubMed
  2. Meng Y,et al. 2017. Cell Death Dis. . 10.1038/cddis.2017.505. PubMed
  3. Murakami T, et al. 2018. Nat Commun. 9:2436. PubMed
  4. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  5. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  6. Yamauchi T et al. 2018. Cancer cell. 33(3):386-400 . PubMed
  7. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  8. Guzzi N et al. 2018. Cell. 173(5):1204-1216 . PubMed
  9. Li CJ, et al. 2018. J Clin Invest. 128:5251. PubMed
  10. Sun YF, et al. 2018. Clin Cancer Res. 24:547. PubMed
  11. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  12. He H, et al. 2019. J Cell Mol Med. 23:4139. PubMed
  13. Salvany‐Celades M et al. 2019. Cell Rep. 27(9):2537-2547 . PubMed
  14. Nasri M, et al. 2019. Haematologica. 104:. PubMed
  15. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  16. Srivastava S, et al. 2019. Cancer Cell. 35:489. PubMed
  17. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  18. Racle J et al. 2017. eLife. 6 pii: e26476. PubMed
  19. de Andrade LF, et al. 2019. JCI Insight. 4:e133103. PubMed
  20. Zhang Y, et al. 2019. Aging (Albany NY). 11:12641. PubMed
  21. Huang X, et al. 2019. Oxid Med Cell Longev. 2019:1305049. PubMed
  22. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  23. Kasagi Y, et al. 2018. Cell Mol Gastroenterol Hepatol. 5:333. PubMed
  24. Hong Y, et al. 2020. Aging Cell. 19:e13128. PubMed
  25. Yang M, et al. 2019. J Exp Med. 216:1944. PubMed
  26. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  27. Giovannone N, et al. 2019. Front Immunol. 2.359027778. PubMed
  28. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  29. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  30. Nagano M, et al. 2007. Blood . 110:151. PubMed
  31. Gleason M, et al. 2014. Blood. 123:3016. PubMed
  32. Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed
  33. Turetsky A, et al. 2014. Sci Rep. 4:4782. PubMed
  34. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  35. O’Dea K, et al. 2016. PLoS One. 11:e0167801. PubMed
  36. Herold N, et al. 2017. Nat Med. 23:256-263. PubMed
  37. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  38. Luzuriaga J, et al. 2020. Biomedicines. 8:00. PubMed
  39. Schmiderer L, et al. 2020. Proc Natl Acad Sci U S A. 117:21267. PubMed
  40. Vignard V, et al. 2020. Cancer Immunol Res. 0.510416667. PubMed
  41. de Jonge K, et al. 2021. OncoImmunology. 10(1):1873585. PubMed
  42. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  43. Hormaechea-Agulla D, et al. 2021. Cell Stem Cell. . PubMed
  44. Subedi A, et al. 2021. Cell Stem Cell. :. PubMed
  45. Gonzalez VD, et al. 2021. Cell Reports. 36(9):109632. PubMed
  46. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  47. Sugimura R, et al. 2020. Biomed Microdevices. 34:22. PubMed
  48. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  49. Hultmark S, et al. 2020. Haematologica. . PubMed
  50. Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed
  51. Weitz JR, et al. 2020. Diabetes. 69:1206. PubMed
  52. Dong R, et al. 2020. EMBO J. 39:e104730. PubMed
  53. Niu LT, et al. 2021. Transl Oncol. 14:101159. PubMed
  54. Wißfeld J, et al. 2021. Glia. 69:1393. PubMed
  55. Shi L, et al. 2021. Stem Cell Res Ther. 12:147. PubMed
  56. Paul S, et al. 2021. Sci Transl Med. 13:. PubMed
  57. Sun YF, et al. 2021. Nat Commun. 12:4091. PubMed
  58. Park JA, et al. 2021. J Hematol Oncol. 14:142. PubMed
  59. Karim F, et al. 2021. Elife. 10:. PubMed
  60. Chen IP, et al. 2020. Bio Protoc. 10:e3854. PubMed
  61. Saygin C, et al. 2021. Leukemia. 35:3406. PubMed
  62. Ruan Y, et al. 2022. Exp Ther Med. 23:47. PubMed
  63. Cortesi EE, et al. 2021. Cells. 10:. PubMed
  64. Vanuytsel K, et al. 2022. Nat Commun. 13:1103. PubMed
  65. Buenafe AC, et al. 2022. Sci Rep. 12:3556. PubMed
  66. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  67. Shao S, et al. 2020. Proc Natl Acad Sci U S A. 117:8845. PubMed
  68. Loreth D, et al. 2021. Int J Mol Sci. 22:. PubMed
  69. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  70. Morita K, et al. 2020. Nat Commun. 11:5327. PubMed
  71. Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
  72. Patel C, et al. 2022. Curr Protoc. 2:e400. PubMed
  73. Delgado-Gonzalez A, et al. 2022. STAR Protoc. 3:101425. PubMed
  74. Bashyal N, et al. 2022. Mol Cells. 45:479. PubMed
  75. Xiao Q, et al. 2022. Sci Immunol. 7:eabl3995. PubMed
  76. Gomez-Gallegos AA, et al. 2023. Stem Cell Res Ther. 14:16. PubMed
  77. Xu QH, et al. 2023. Am J Reprod Immunol. 89:e13581. PubMed
  78. Michalek S, et al. 2022. EMBO Rep. 23:e54195. PubMed
  79. Klawitter M, et al. 2022. Int J Mol Sci. 23:. PubMed
  80. Siepe DH, et al. 2022. Elife. 11:. PubMed
  81. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  82. Sun J, et al. 2023. Oncogene. 42:1247. PubMed
  83. Xiao Y, et al. 2023. EMBO J. 42:e111762. PubMed
RRID
AB_314399 (BioLegend Cat. No. 304011)
AB_314400 (BioLegend Cat. No. 304012)
AB_2562049 (BioLegend Cat. No. 304037)

Antigen Details

Structure
Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)
Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function
TCR and BCR mediated activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
Hematopoietic stem and progenitors, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References
  1. Thomas M. 1989. Annu. Rev. Immunol. 7:339.
  2. Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.
Gene ID
5788 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org
Go To Top Version: 2    Revision Date: 04.25.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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