Loading...

APC anti-human CD56 (NCAM) Antibody

Pricing & Availability
Clone
HCD56 (See other available formats)
Regulatory Status
RUO
Other Names
Leu-19, NKH1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HCD56_APC_020808
Human peripheral blood lymphocytes stained with HCD56 APC
  • HCD56_APC_020808
    Human peripheral blood lymphocytes stained with HCD56 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
318309 25 tests 120€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
318310 100 tests 252€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD56 is a single transmembrane glycoprotein also known as NCAM (Neural Cell Adhesion Molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK cells and NK-T cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparin sulfate.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HCD56 is not recommended for immunohistochemistry formalin-fixed paraffin-embedded tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  2. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Turaj AH et al. 2017. Cancer cell. 32(6):777-791 . PubMed
  2. Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
  3. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  4. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  5. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  6. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  7. Smith SA, et al. 2019. Front Immunol. 9:3163. PubMed
  8. Taouk G, et al. 2019. Sci Rep. 9:8756. PubMed
  9. Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed
  10. Miguel Reina‐Campos et al. 2019. Cancer cell. 35(3):385-400 . PubMed
  11. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  12. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  13. Ma Y, et al. 2020. Cell Prolif. 53:e12802. PubMed
  14. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  15. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  16. Pakula A, et al. 2018. Methods Mol Biol. 1889:01:00. PubMed
  17. Rosental B, et al. 2011. J Immunol. 187:5693. PubMed
  18. Correia DV, et al. 2011. Blood. 118:992. PubMed
  19. Schlecker E, et al. 2014. Cancer Res. 74:3429. PubMed
  20. Snyder J, et al. 2014. PLoS One. 9:107257. PubMed
  21. Siebert N, et al. 2014. PLoS One. 9:107692. PubMed
  22. Rider P, et al. 2015. J Immunol. 195: 1705-1712. PubMed
  23. Davis Z, et al. 2011. J Vis Exp. 49: 2668. PubMed
  24. Robin J, et al. 2015. J Vis Exp. 95: 52307. PubMed
  25. Ames E, et al. 2015. J Immunol. 195: 4010 - 4019. PubMed
  26. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  27. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  28. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  29. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  30. Al Tanoury Z, et al. 2020. Development. 147:00:00. PubMed
  31. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  32. Yan W, et al. 2020. Biology of Reproduction. . PubMed
  33. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  34. Abdul-Jawad S, et al. 2021. Cancer Cell. 39(2):257-275.e6. PubMed
  35. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  36. Andersen J, et al. 2020. Cell. 183:1913. PubMed
  37. Kan S, et al. 2020. Int J Oncol. 57:1047. PubMed
  38. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  39. Strijker JGM, et al. 2021. J Pers Med. 11:. PubMed
  40. Al Tanoury Z, et al. 2020. Development. . PubMed
  41. Molina MS, et al. 2021. Front Immunol. 12:699128. PubMed
  42. Ramendra R, et al. 2021. Front Immunol. 12:694152. PubMed
  43. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  44. Yang Zhou J, et al. 2022. Front Immunol. 13:898473. PubMed
  45. Vabret N, et al. 2022. iScience. 25:104599. PubMed
  46. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  47. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  48. Pereira PMR, et al. 2022. Nat Commun. 13:2526. PubMed
  49. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  50. Kenney DJ, et al. 2022. Cell Rep. 39:110714. PubMed
  51. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  52. Chen L, et al. 2023. Immunology. 169:204. PubMed
  53. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
RRID
AB_604098 (BioLegend Cat. No. 318309)
AB_604106 (BioLegend Cat. No. 318310)

Antigen Details

Structure
Ig superfamily, single transmembrane or GPI-anchored glycoprotein
Distribution

NK cells, T subset, neural tissue, some LGL and myeloid leukemias

Function
Adhesion
Ligand/Receptor
Heparin sulfate
Cell Type
B cells, Leukemia, Mesenchymal Stem Cells, Neurons, NK cells, T cells
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Lanier L, et al. 1991. J. Immunol. 146:4421.
2. Hemperly J, et al. 1990. J. Mol. Neurosci. 2:71.
3. Cremer H, et al. 1994. Nature 367:455.

Gene ID
4684 View all products for this Gene ID
UniProt
View information about CD56 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account