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APC anti-human CD81 (TAPA-1) Antibody

Pricing & Availability
Clone
5A6 (See other available formats)
Regulatory Status
RUO
Other Names
S5.7, CVID6, TSPAN28
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5A6_APC_CD81_Antibody_FC_081214.jpg
Human peripheral blood lymphocytes were stained with anti-human CD81 (clone 5A6) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 5A6_APC_CD81_Antibody_FC_081214.jpg
    Human peripheral blood lymphocytes were stained with anti-human CD81 (clone 5A6) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Check Availability Save
349509 25 tests 86€
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349510 100 tests 247€
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Description

CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expression on granulocytes. CD81 induces B cell adhesion via VLA-4 integrin and has been shown to play a role in early T cell development. CD81 associates with several other cell-surface proteins in a multimolecular complex, including CD19, CD21, CD20, CD37, CD53, and CD82 in B cells, and CD4, CD8, and CD82 in T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human OCI-LY8 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western Blotting3 and immunoprecipitation2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Oren R, et al. 1990. Mol. Cell. Biol. 8:4007. (IP)
  3. Clark K, et al. 2004. J. Biol. Chem. 279(19):19401. (IP, WB)
  4. Mochida K, et al. 2008. J. Virol. 13:6711.
  5. Rappa G, et al. 2014. Mol Cancer Res. 12:1840. PubMed
Product Citations
  1. Najm FJ, et al. 2018. Nat Biotechnol. 36:179. PubMed
  2. Zimmerman B et al. 2016. Cell. 167(4):1041-1051 . PubMed
  3. Angeloni N, et al. 2016. Sci Rep. 6:22915. PubMed
  4. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  5. Ragni E, et al. 2020. Stem Cell Res Ther. 165:11. PubMed
  6. Boruchowicz H, et al. 2020. J Virol. :94. PubMed
  7. Ragni E, et al. 2021. Cells. 10: . PubMed
  8. Samelson AJ, et al. 2022. Nat Cell Biol. 24:24. PubMed
  9. Ragni E, et al. 2022. Pharmaceutics. 14:. PubMed
  10. Papait A, et al. 2022. Front Immunol. 13:960909. PubMed
  11. Alerasool N, et al. 2020. Nat Methods. 17:1093. PubMed
  12. Ragni E, et al. 2022. Biomolecules. 12:. PubMed
  13. Abe I, et al. 2022. Dev Cell. 57:2623. PubMed
  14. Kocak P, et al. 2023. Aesthet Surg J Open Forum. 5:ojad039. PubMed
RRID
AB_2564020 (BioLegend Cat. No. 349509)
AB_2564021 (BioLegend Cat. No. 349510)

Antigen Details

Structure
26 kD, type III transmembrane protein, member of the TM4SF tetraspanin family. Complexed with CD82, CD19, CD21, or CD4, CD8.
Distribution

T cells, NK, monocytes, B cells, endothelial and epithelial cells, low on granulocytes.

Function
Regulates cell activation and growth and cell aggregation.
Cell Type
B cells, Endothelial cells, Epithelial cells, Monocytes, Neural Stem Cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells, Transcription Factors
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Van Zelm MC, et al. 2010. J. Clin. Invest. 120:1265.
  2. Fearon D, et al. 1995. Annu. Rev. Immunol. 13:127.
  3. Wright M, et al. 1994. Immunol. Today 15:588.
Gene ID
975 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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