- Clone
- GoH3 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- VLA-6 α chain, α6 integrin, integrin α6, ITGA6
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![GOH3_APC_102110 GOH3_APC_102110](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/GOH3_APC_102110.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with anti-human/mouse CD49f (clone GOH3) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
313615 | 25 tests | 120€ | ||||
313616 | 100 tests | 252€ |
CD49f is a 120 kD integrin family member also known as VLA-6 α chain and α6 integrin subunit. CD49f associates with either integrin β1 (CD29) or integrin β4 (CD104) to form receptors (VLA-6 or α6β4 complex) for laminin and kalinin. CD49f is expressed on platelets, monocytes, T cells, placental trophoblasts, and epithelial and endothelial cells. CD49f is involved in adhesion and can act as a co-stimulatory molecule for T cell activation and proliferation.
Product DetailsProduct Details
- Reactivity
- Human,Mouse,Cynomolgus,Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse mammary tumor cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: immunoprecipitation1,5, in vitro and in vivo blocking of cell binding to laminin and blocking the function of integrin a61,4, and immunohistochemistry of acetone-fixed frozen sections2,3,5. The GoH3 antibody has been reported to block laminin binding in vitro and to block integrin a6 function in vivo.
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Application References
(PubMed link indicates BioLegend citation) -
- Georas SN, et al. 1993. Blood 82:2872. (IP, Block)
- Honda T, et al. 1995. J. Clin. Endocrinol. Metab. 80:2899. (IHC)
- Sonnenberg A, et al. 1986. J. Histochem. Cytochem. 34:1037. (IHC)
- Nakamura K, et al. 1997 Biochem. Biophys. Res. Commun. 235:524. (Block)
- Sonnenberg A, et al. 1987 J. Biol. Chem. 262:10376. (IP, IHC)
- Deregibus MC, et al. 2007. Blood doi:10.1182/blood-2007-03-078709.
- Horwitz KB, et al. 2008. Proc Natl Acad Sci USA. 105:5774. PubMed
- Nardella C, et al. 2009. Sci Signal. 2:55. PubMed
- Xu T, et al. 2010. Mol Cancer Ther. 9:438. PubMed
- Stepp MA, et al. 2007. J Cell Sci. 120:2851. PubMed
- Jo M, et al. 2010. Cancer Res. 70:8948. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Grange C, et al. 2011. Cancer Res. 71:5346. PubMed
- Lai KP, et al. 2012. Mol Endocrinol. 26:52. PubMed
- Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
- Product Citations
- RRID
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AB_1575047 (BioLegend Cat. No. 313615)
AB_1575047 (BioLegend Cat. No. 313616)
Antigen Details
- Structure
- Integrin family, associates with β1 or β4, 120 kD
- Distribution
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Platelets, monocytes, T cells, placental trophoblasts, epithelial and endothelial cells
- Function
- Adhesion, receptor for laminin and kalinin; laminin binding to VLA-6 induces T cell co-stimulation for proliferation and activation
- Ligand/Receptor
- With integrin β1 (CD29) forms VLA-6, with integrin β4 (CD104) forms a6β4 integrin; laminin and kalinin are ligands for these receptors
- Cell Type
- Embryonic Stem Cells, Endothelial cells, Epithelial cells, Monocytes, Platelets, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Sonnenberg A, et al. 1990. J. Cell Biol. 110:2145.
2. Sonnenberg A, et al. 1990. J. Cell. Sci. 96:207.
3. Aumailley M, et al. 1990. Exp. Cell Res. 188:55.
4. Niessen CM, et al. 1994. Exp. Cell Res. 211:360. - Gene ID
- 16403 View all products for this Gene ID 3655 View all products for this Gene ID
- UniProt
- View information about CD49f on UniProt.org
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