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APC anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Regulatory Status
RUO
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
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Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
See APC spectral data
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118213 25 µg 82€
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118214 100 µg 237€
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  14. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Chua CW, et al. 2018. Elife. 7. PubMed
  2. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  3. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  4. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  5. Malik A et al. 2018. Immunity. 49(3):515-530 . PubMed
  6. Cohen I et al. 2018. Cell stem cell. 22(5):726-739 . PubMed
  7. Renz BW, et al. 2018. Cancer Cell. 33:75. PubMed
  8. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  9. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
  10. Bar C, et al. 2020. Cell Reports. 28(1):257-266.e5.. PubMed
  11. Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
  12. Kato T, et al. 2019. Front Immunol. 10:2021. PubMed
  13. Pepe‐Mooney BJ et al. 2019. Cell Stem Cell. 25(1):23-38 . PubMed
  14. Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
  15. Castillo–Azofeifa D, et al. 2019. EMBO J. 38:e99984. PubMed
  16. Katara GK, et al. 2020. Mol Oncol. 0.729861111. PubMed
  17. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  18. Jadhav U, et al. 2019. Mol Cell. 74:542. PubMed
  19. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  20. Sateriale A, et al. 2020. Cell Host & Microbe. 26(1):135-146.e5.. PubMed
  21. Chen Q, et al. 2020. Cell Reports. 31(13):107828. PubMed
  22. Liu S, et al. 2020. Cell Host & Microbe. 26(6):779-794.e8.. PubMed
  23. Han H, et al. 2020. Cancer Cell. 36(5):483-497.e15.. PubMed
  24. McGinty JW, et al. 2020. Immunity. 52(3):528-541. PubMed
  25. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  26. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  27. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  28. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  29. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  30. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  31. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  32. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  33. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  34. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  35. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  36. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  37. Mevel R, et al. 2020. eLife. 9:00. PubMed
  38. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  39. Paris AJ, et al. 2020. Nat Cell Biol. 1.747916667. PubMed
  40. Caporarello N, et al. 2020. Aging Cell. 19:e13196. PubMed
  41. Christin JR, et al. 2020. Cell Reports. 31(10):107742. PubMed
  42. Phung HT, et al. 2020. Immunohorizons. 0.25625. PubMed
  43. Ludwik KA, et al. 2021. STAR Protocols. 2(1):100270. PubMed
  44. Bowling S, et al. 2020. Cell. 181(6):1410-1422.e27. PubMed
  45. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
  46. Chen Z, et al. 2020. Immunity. 54(1):151-163.e6. PubMed
  47. Jiang M, et al. 2021. Developmental Cell. 56(11):1646-1660.e5. PubMed
  48. Li MY, et al. 2021. Developmental Cell. 56:2547. PubMed
  49. Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed
  50. Kasuga A, et al. 2020. Cancer Sci. . PubMed
  51. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  52. Hertzano R, et al. 2020. Laryngoscope. . PubMed
  53. Semba T, et al. 2020. Cancers (Basel). 12:. PubMed
  54. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  55. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  56. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  57. Tang Z, et al. 2021. Stem Cell Res Ther. 12:397. PubMed
  58. Ford MJ, et al. 2021. Cell Rep. 36:109677. PubMed
  59. Cerretelli G, et al. 2021. J Pathol. 255:464. PubMed
  60. Tuong ZK, et al. 2021. iScience. 24:103326. PubMed
  61. Ognjenovic NB, et al. 2020. Dev Cell. 55:544. PubMed
  62. Willobee BA, et al. 2021. Mol Cancer Ther. 20:1246. PubMed
  63. Li J, et al. 2020. Development. :. PubMed
  64. Rudnick JA, et al. 2021. Genes Dev. 35:963. PubMed
  65. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
  66. Lv X, et al. 2022. Acta Pharm Sin B. 12:735. PubMed
  67. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  68. Nishina T, et al. 2021. Nat Commun. 12:2281. PubMed
  69. Wu H, et al. 2021. Cell Death Discov. 7:225. PubMed
  70. Goldfarb Y, et al. 2021. J Exp Med. 218:. PubMed
  71. Ionkina AA, et al. 2021. Breast Cancer Res. 23:93. PubMed
  72. Caporarello N, et al. 2022. Nat Commun. 13:4170. PubMed
  73. Zhu Y, et al. 2022. Nat Commun. 13:4282. PubMed
  74. Lehmann M, et al. 2020. Cell Signal. 70:109588. PubMed
  75. Zhang Z, et al. 2021. Exp Dermatol. 30:102. PubMed
  76. Ualiyeva S, et al. 2021. Bio Protoc. 11:e4163. PubMed
  77. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  78. Park SE, et al. 2022. Cell Mol Gastroenterol Hepatol. 14:1235. PubMed
  79. Na E, et al. 2022. Am J Physiol Lung Cell Mol Physiol. 322:L550. PubMed
  80. Xu J, et al. 2022. Dev Cell. 57:839. PubMed
  81. Moriyama M, et al. 2023. Proc Natl Acad Sci U S A. 120:e2221652120. PubMed
  82. Lv X, et al. 2023. Acta Pharm Sin B. 13:1631. PubMed
  83. Cui W, et al. 2023. J Exp Med. 220:. PubMed
  84. Kalgudde Gopal S, et al. 2023. Nat Commun. 14:3020. PubMed
RRID
AB_1134105 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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