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Brilliant Violet 421™ anti-human CD335 (NKp46) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9E2_BV421_1_110811
Human peripheral blood lymphocytes were stained with CD56 APC and CD335 (clone 9E2) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 9E2_BV421_1_110811
    Human peripheral blood lymphocytes were stained with CD56 APC and CD335 (clone 9E2) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 9E2_BV421_2_110811
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
331913 25 tests 172€
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331914 100 tests 340€
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Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
Product Citations
  1. Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
  2. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
  3. Antonarakis ES, et al. 2018. Clin Cancer Res. 24:4662. PubMed
  4. Heger L, et al. 2018. Front Immunol. 9:744. PubMed
  5. Lee J, et al. 2015. J Exp Med. 212:385. PubMed
  6. Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
  7. Maas RJ, et al. 2020. Oncoimmunology. 9:1843247. PubMed
  8. Apte SH, et al. 2020. Clin Transl Immunology. 9:e1209. PubMed
  9. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  10. Alonso R, et al. 2022. Blood Adv. :. PubMed
  11. Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed
RRID
AB_10916529 (BioLegend Cat. No. 331913)
AB_2563853 (BioLegend Cat. No. 331914)

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 04.15.2014

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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