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Brilliant Violet 421™ anti-human CD366 (Tim-3) Antibody

Pricing & Availability
Clone
F38-2E2 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
F38-2E2_BV421_072711
Th1-polarized human peripheral blood lymphocytes were stained with anti-human CD366 (Tim-3, clone F38-2E2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
  • F38-2E2_BV421_072711
    Th1-polarized human peripheral blood lymphocytes were stained with anti-human CD366 (Tim-3, clone F38-2E2) Brilliant Violet 421™ (filled histogram) or mouse IgG1, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
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345007 25 tests 172€
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345008 100 tests 326€
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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Tim-3 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
  2. Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
  3. Klibi J, et al 2009. Blood 113:1957. (FC, Block)
Product Citations
  1. Cunningham RA, et al. 2019. J Biol Methods. 6:e114. PubMed
  2. Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed
  3. Li X, et al. 2019. Front Oncol. 1.115277778. PubMed
  4. Kivit S, et al. 2015. Clin Immunol. 156:28. PubMed
  5. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  6. Thommen D, et al. 2015. Cancer Immunol Res. 3: 1344 - 1355. PubMed
  7. Trivedi S, et al. 2020. Elife. 9:00. PubMed
  8. Wenthe J, et al. 2021. Cancer Immunology Immunotherapy. . PubMed
  9. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  10. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  11. Brudno JN, et al. 2020. Nat Med. 270:26. PubMed
  12. Fan P, et al. 2021. Front Oncol. 11:678758. PubMed
  13. Wang S, et al. 2021. Oncol Rep. 1.91666666666667. PubMed
  14. Junker F, et al. 2021. Cytometry A. 99:832. PubMed
  15. Gamradt S, et al. 2021. iScience. 24:103312. PubMed
  16. Carnevale J, et al. 2022. Nature. 609:174. PubMed
  17. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  18. Hsiao CC, et al. 2023. iScience. 26:105785. PubMed
  19. Labuz DR, et al. 2023. Elife. 12: . PubMed
  20. Imai H, et al. 2023. iScience. 26:106822. PubMed
RRID
AB_10900073 (BioLegend Cat. No. 345007)
AB_11218598 (BioLegend Cat. No. 345008)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells

Function
Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor
Galectin-9
Cell Type
Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.

Gene ID
84868 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 5    Revision Date: 07.03.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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