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Brilliant Violet 510™ anti-mouse CD19 Antibody

Pricing & Availability
Clone
6D5 (See other available formats)
Regulatory Status
RUO
Other Names
B4
Isotype
Rat IgG2a, κ
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Product Citations
publications
6D5_BV510_CD19_Antibody_FC_051413
C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ control (open histogram).
  • 6D5_BV510_CD19_Antibody_FC_051413
    C57BL/6 mouse splenocytes were stained with CD19 (clone 6D5) Brilliant Violet 510™ (filled histogram) or rat IgG2a, κ Brilliant Violet 510™ control (open histogram).
See Brilliant Violet 510™ spectral data
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115545 125 µL 132€
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115546 500 µL 308€
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Description

CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD19-expressing K562 human erythroleukemia cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence7.

Application References

(PubMed link indicates BioLegend citation)
  1. Shoham T, et al. 2003. J. Immunol. 171:4062. (FC)
  2. Goodyear CS, et al. 2004. J. Immunol. 172:2870. (FC)
  3. Kamimura D, et al. 2006. J. Immunol. 177:306. (FC)
  4. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (FC)
  5. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  6. Phan TG, et al. 2007. Nat. Immunol. 8:992. (FC)
  7. Hayashida K, et al. 2008. J. Biol. Chem. 283:19895. (IF) PubMed
  8. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  9. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  10. Stadnisky MD, et al. 2011. Blood. 117:5133. (FC) PubMed
  11. Perlot T, et al. 2012. J. Immunol. 188:1201. (FC) PubMed
  12. Olive V, et al. 2013. Elife. 2:822. PubMed
  13. Miyai T, et al. 2014. PNAS. 111:11780. PubMed
Product Citations
  1. Landon J Edgar et al. 2018. Cell chemical biology. 26(1):131-136 . PubMed
  2. Rydyznski CE et al. 2018. Cell reports. 24(13):3367-3373 . PubMed
  3. Barbet G, et al. 2018. Immunity. 48:584. PubMed
  4. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  5. Williams JB, et al. 2020. Nat Commun. 0.876388889. PubMed
  6. Zou D, et al. 2020. EBioMedicine. 52:102652. PubMed
  7. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  8. Hartmann W, et al. 2020. Cell Reports. 29(8):2243-2256.e4.. PubMed
  9. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  10. Alterauge D, et al. 2020. Cell Rep. 33:108232. PubMed
  11. Santopaolo M, et al. 2021. Front Immunol. 609406:12. PubMed
  12. Werner A, et al. 2021. iScience. 24:103076. PubMed
  13. Woodworth JS, et al. 2021. Nat Commun. 12:6658. PubMed
  14. Bhatt D, et al. 2021. J Exp Med. 218:. PubMed
  15. Sauter M, et al. 2022. iScience. 25:103677. PubMed
  16. Hu M, et al. 2020. Cancer Immunol Res. 8:1150. PubMed
  17. Mills RH, et al. 2022. Nat Microbiol. 7:262. PubMed
  18. Flickinger JC, et al. 2022. NPJ Vaccines. 7:61. PubMed
  19. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  20. Campisi L, et al. 2022. Nature. 606:945. PubMed
  21. Potempa M, et al. 2022. J Immunol. 208:1742. PubMed
  22. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  23. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  24. Ludwig J, et al. 2023. NPJ Vaccines. 8:52. PubMed
  25. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  26. Dijkman K, et al. 2023. NPJ Vaccines. 8:66. PubMed
  27. Borbet TC, et al. 2023. iScience. 26:106810. PubMed
RRID
AB_2562136 (BioLegend Cat. No. 115545)
AB_2562137 (BioLegend Cat. No. 115546)

Antigen Details

Structure
Ig superfamily, associates with CD21 and CD81, 95 kD
Distribution

Pro-B cells to mature B cells (during development), follicular dendritic cells

Function
Modulates B cell activation and differentiation
Ligand/Receptor
CD21, CD81, Leu-13
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437.

Gene ID
12478 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 2    Revision Date: 05.24.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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