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Brilliant Violet 785™ anti-mouse CD90.2 (Thy1.2) Antibody

Pricing & Availability
Clone
30-H12 (See other available formats)
Regulatory Status
RUO
Other Names
Thy-1.2
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
30-H12_BV785_CD90.2_Antibody_FC_120213
C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) Brilliant Violet 785™ (filled histogram) or rat IgG2b, κ Brilliant Violet 785™ isotype control (open histogram).
  • 30-H12_BV785_CD90.2_Antibody_FC_120213
    C57BL/6 thymocytes were stained with CD90.2 (clone 30-H12) Brilliant Violet 785™ (filled histogram) or rat IgG2b, κ Brilliant Violet 785™ isotype control (open histogram).
See Brilliant Violet 785™ spectral data
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105331 50 µg 248€
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Description

CD90.2 is a 25-35 kD immunoglobulin superfamily member also known as Thy1.2. It is expressed on hematopoietic stem cells and neurons, all thymocytes, and peripheral T cells in Thy1.2 bearing mouse strains (Balb/c, CBA/J, C3H/He, C57BL/-, DBA, NZB/-). CD90.2 is a glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein involved in signal transduction. CD90.2 is involved in costimulation of lymphocyte proliferation and induction of hematopoietic stem cells differentiation. CD90.2 has been shown to interact with CD45. The 30-H12 antibody has been reported to induce Ca2+ flux in thymocytes and, in combination with antibody against the CD3/TCR complex, promote thymocyte apoptosis and inhibit CD3-mediated proliferative responses of mature T lymphocytes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro depletion1,2,7, costimulation of CD3/TCR-mediated signal transduction3,4, and immunohistochemical staining5 of acetone-fixed frozen sections. The 30-H12 antibody does not react with Thy-1.1 alloantigen of the AKR/J and PL strains. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301 & 101302) is recommended prior to immunofluorescent staining. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 105351 & 105352).

Application References

(PubMed link indicates BioLegend citation)
  1. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  2. Seaman WE. 1983. J. Immunol. 130:1713. (Deplete)
  3. Nakashima I, et al. 1991. J. Immunol. 147:1153. (Costim)
  4. Nakashima I, et al. 1993. J. Immunol. 151:3511. (Costim)
  5. Ledbetter JA, et al. 1980. J. Exp. Med. 152:280. (IHC)
  6. Hardy B, et al. 2005. Int. Immunol. 17:615.
  7. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  8. Dyer KD, et al. 2007. J. Immunol. 179:1693. (FC) PubMed
  9. Sungur CM, et al. 2013. PNAS. 110:7401. PubMed
Product Citations
  1. Crinier A, et al. 2018. Immunity. 49:971. PubMed
  2. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  3. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  4. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  5. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
  6. Barry KC, et al. 2018. Nat Med. 24:1178. PubMed
  7. Schneider C, et al. 2018. Cell. 174:271. PubMed
  8. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  9. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  10. Baryawno N et al. 2019. Cell. 177(7):1915-1932 . PubMed
  11. Zhou Y, et al. 2020. Immunity. 52(2):357-373. PubMed
  12. Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
  13. Hering L, et al. 2020. Front Immunol. 1.747222222. PubMed
  14. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  15. Borges da Silva H, et al. 2020. Immunity. 53(1):158-171.e6. PubMed
  16. Huang X, et al. 2020. Cell Host Microbe. 29(2):210-221.e6. PubMed
  17. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  18. Lima-Junior DS, et al. 2021. Cell. . PubMed
  19. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  20. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  21. Kinsella S, et al. 2021. Cell Rep. 37:109789. PubMed
  22. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  23. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  24. Mujal AM, et al. 2022. Cancer Immunol Res. 10:403. PubMed
  25. Kohn EM, et al. 2022. J Immunol. 208:1417. PubMed
  26. Kedage V, et al. 2022. MAbs. 14:2040083. PubMed
  27. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  28. Kuczynski EA, et al. 2022. EMBO Mol Med. 14:e15816. PubMed
RRID
AB_2562900 (BioLegend Cat. No. 105331)

Antigen Details

Structure
Ig superfamily, 25-35 kD
Distribution

Hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice

Function
Lymphocyte costimulation, proliferation and differentiation of hematopoietic stem cells
Ligand/Receptor
CD45
Cell Type
Hematopoietic stem and progenitors, Neurons, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Craig W, et al. 1993. J. Exp. Med. 177:1331.
3. Reif AE and Schlesinger M. 1989. Cell Surface Antigen Thy-1.
4. Mayani H, et al. 1994. Blood 83:2410.

Gene ID
21838 View all products for this Gene ID
UniProt
View information about CD90.2 on UniProt.org
Go To Top Version: 1    Revision Date: 12.13.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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