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FITC anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK7_FITC_021810
Human peripheral blood lymphocytes stained with SK7 FITC.
  • SK7_FITC_021810
    Human peripheral blood lymphocytes stained with SK7 FITC.
See FITC spectral data
Cat # Size Price Quantity Check Availability Save
344803 25 tests 58€
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344804 100 tests 128€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Chheda ZS, et al. 2018. J Exp Med. 9:19490. PubMed
  2. Hagan T, et al. 2020. Cell. 178(6):1313-1328.e13.. PubMed
  3. Carter JA, et al. 2020. Cell Systems. 9(5):475-482.e4.. PubMed
  4. Björklund &, et al. 2016. Nat Immunol. 17:451-460. PubMed
  5. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  6. Montes de Oca M, et al. 2016. Cell Rep. 17:399-412. PubMed
  7. Cao M, et al. 2020. Aging Dis. 1.479861111. PubMed
  8. Penkava F, et al. 2020. Nat Commun. 3.76875. PubMed
  9. Roudko V, et al. 2020. Cell. 183(6):1634-1649.e17. PubMed
  10. Thiel BA, et al. 2021. PLoS One. 16:e0249477. PubMed
  11. Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
  12. Prota G, et al. 2020. Cancer Immunol Res. 8:685. PubMed
  13. Qing G, et al. 2021. Front Cell Dev Biol. 9:761300. PubMed
  14. Mudd PA, et al. 2022. Cell. 185:603. PubMed
  15. Chan S, et al. 2022. Nat Cancer. . PubMed
  16. Cimen Bozkus C, et al. 2021. STAR Protoc. 2:100758. PubMed
  17. Chan JA, et al. 2022. Nat Commun. 13:4159. PubMed
  18. Nagree MS, et al. 2022. EMBO Mol Med. 14:e14297. PubMed
  19. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  20. Weaver JD, et al. 2022. Oncoimmunology. 11:2141007. PubMed
  21. Kuloglu ZE, et al. 2022. Allergy. 77:2459. PubMed
  22. Hu Z, et al. 2022. Nat Metab. 4:759. PubMed
  23. Hong Y, et al. 2022. J Leukoc Biol. 112:425. PubMed
  24. Balta E, et al. 2023. Front Immunol. 13:1063313. PubMed
RRID
AB_2043992 (BioLegend Cat. No. 344803)
AB_2043993 (BioLegend Cat. No. 344804)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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