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FITC anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_FITC_092409
Human peripheral blood lymphocytes stained with SK1 FITC
  • SK1_FITC_092409
    Human peripheral blood lymphocytes stained with SK1 FITC
See FITC spectral data
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344703 25 tests 97€
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344704 100 tests 194€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  2. Zuazo M, et al. 2019. EMBO Mol Med. 11(7). PubMed
  3. Lou Q, et al. 2019. J Immunother Cancer. 7:210. PubMed
  4. Leng T, et al. 2019. Cell Rep. 28:3077. PubMed
  5. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  6. Wang Z, et al. 2019. Cell Mol Life Sci. :. PubMed
  7. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  8. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  9. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  10. Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
  11. Sun B, et al. 2019. Cancer Immunol Res. 1.550694444. PubMed
  12. Martínez-Fábregas J, et al. 2020. Cell Rep. 33:108545. PubMed
  13. Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
  14. Barresi V, et al. 2020. J Clin Med. 9:00. PubMed
  15. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  16. Gannon PO, et al. 2020. Cytotherapy. 22(12):780-791. PubMed
  17. Lu C, et al. 2020. Cancer Cell. 39(1):96-108.e6. PubMed
  18. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  19. Goc J, et al. 2021. Cell. 184:5015. PubMed
  20. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  21. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  22. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  23. Méndez-Lagares G, et al. 2021. J Clin Invest. 131:e148542. PubMed
  24. Gullà A, et al. 2021. Cancer Discov. 2:468. PubMed
  25. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  26. Prota G, et al. 2020. Cancer Immunol Res. 8:685. PubMed
  27. Gullà A, et al. 2021. Cancer Discov. . PubMed
  28. Guo J, et al. 2022. Front Cell Dev Biol. 9:775599. PubMed
  29. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  30. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  31. Xu C, et al. 2020. Methods Mol Biol. 2108:159. PubMed
  32. Hess NJ, et al. 2020. J Immunol. 205:272. PubMed
  33. Hu Q, et al. 2021. Nat Biomed Eng. 5:1038. PubMed
  34. Weiss R, et al. 2021. Cells. 10:. PubMed
  35. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  36. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  37. Woan KV, et al. 2021. Cell Stem Cell. 28:2062. PubMed
  38. Anadon CM, et al. 2022. Cancer Cell. 40:545. PubMed
  39. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  40. Le Y, et al. 2022. iScience. 25:105426. PubMed
  41. Liu C, et al. 2022. Front Immunol. 13:1039631. PubMed
  42. Peng W, et al. 2023. Cells. 12: . PubMed
  43. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  44. Liao Y, et al. 2023. Am J Transl Res. 15:817. PubMed
  45. Genolet R, et al. 2023. Cell Rep Methods. 3:100459. PubMed
RRID
AB_1877179 (BioLegend Cat. No. 344703)
AB_1877178 (BioLegend Cat. No. 344704)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org
Go To Top Version: 4    Revision Date: 07.13.2015

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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