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FITC anti-human IgD Antibody

Pricing & Availability
Clone
IA6-2 (See other available formats)
Regulatory Status
RUO
Other Names
Ig delta chain C region
Isotype
Mouse IgG2a, κ
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Product Citations
publications
IA6-2_FITC_IGd_Antibody_1_FC_090216
  • IA6-2_FITC_IGd_Antibody_1_FC_090216
  • IA6-2_FITC_IGd_Antibody_2_FC_090216
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348205 25 tests 87€
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348206 100 tests 202€
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Description

IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IgD
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen K, et al. 2009. Nat. Immunol. 10:889.
  2. Lee CH, et al. 2005. J. Exp. Med. 203:63.
  3. Sutter JA, et al. 2008. Clin. Immunol. 126:282.
  4. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Klymenko T, et al. 2018. Leukemia. 19:E499. PubMed
  2. van der Burg M, et al. 2019. Front Immunol. 10:246. PubMed
  3. Munteanu AN, et al. 2019. Exp Ther Med. 18:1693. PubMed
  4. van Dongen JJM, et al. 2019. Front Immunol. 10:1271. PubMed
  5. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  6. Pallikkuth S, et al. 2020. eLife. 9:e51889.. PubMed
  7. Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed
  8. Heiden M, et al. 2016. Sci Rep. 6: 26892. PubMed
  9. Rother M, et al. 2016. J Immunol. 197: 441 - 448. PubMed
  10. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  11. Wu X, et al. 2016. Sci Rep. 6:36378. PubMed
  12. van der Velden V, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.011. PubMed
  13. Zost SJ, et al. 2020. Nat Med. 26:1422. PubMed
  14. Mukhamedova M, et al. 2021. Immunity. 54(4):769-780.e6. PubMed
  15. Fu Y, et al. 2021. Cell Reports. 36(2):109344. PubMed
  16. Tong P, et al. 2021. Cell. :. PubMed
  17. Zost SJ, et al. 2020. bioRxiv. . PubMed
  18. Benhammou JN, et al. 2020. Dig Dis Sci. 66:2394. PubMed
  19. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  20. Nakano M, et al. 2021. Front Immunol. 12:713225. PubMed
  21. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  22. Jiang R, et al. 2020. JCI Insight. 5:. PubMed
  23. SoRelle ED, et al. 2022. Cell Rep. 40:111286. PubMed
  24. Donnadieu E, et al. 2020. J Immunol. 205:1248. PubMed
  25. Schneider MM, et al. 2022. Life Sci Alliance. 5: . PubMed
  26. Nagashima K, et al. 2022. J Immunol. 209:5. PubMed
  27. Hossain MA, et al. 2022. Cell Rep. 39:111021. PubMed
  28. Parry H, et al. 2022. Cell Rep Med. 3:100739. PubMed
  29. Shitaoka K, et al. 2023. Commun Biol. 6:395. PubMed
  30. García-Vega M, et al. 2023. iScience. 26:106562. PubMed
  31. Wu X, et al. 2023. iScience. 26:106559. PubMed
RRID
AB_10613638 (BioLegend Cat. No. 348205)
AB_10612567 (BioLegend Cat. No. 348206)

Antigen Details

Structure
Exists in a transmembranal and a soluble form
Distribution

Naïve B cells

Function
Antigen binding, B cell activation
Interaction
The CD79a/CD79b heterodimer
Cell Type
B cells
Biology Area
Immunology
Antigen References

1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.

Gene ID
3495 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

There are no FAQs for this product.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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