- Clone
- GB11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Granzyme-2, serine protease B, CCP1, Asp-ase, CTLA-1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with GB11 FITC -
Human peripheral blood lymphocytes surface stained with CD8 PE, then intracellular stained with mouse IgG1 FITC isotype control
Cat # | Size | Price | Quantity Check Availability | Save | ||
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515403 | 25 tests | 108€ |
Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Wever PC, et al. 1998. Immunology. 93:383
- Arens R, et al. 2004. J. Exp. Med. 199:1595
- Lima M, et al. 2003. Am. J. Pathol. 163:763
- Wiede F, et al. 2014. J Autoimmun. 53:105. PubMed
- Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
- Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
- Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
- Product Citations
- RRID
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AB_2114575 (BioLegend Cat. No. 515403)
Antigen Details
- Structure
- 32 kD serine protease
- Distribution
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Cytotoxic T-cells and NK cells, low on granulocytes, B cells and activated dendritic cells
- Function
- Induction of cell death and apoptosis
- Ligand/Receptor
- Mannose-6-phosphate receptor
- Cell Type
- B cells, Dendritic cells, NK cells, T cells
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Enzymes and Regulators, Proteases
- Antigen References
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1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761 - Gene ID
- 3002 View all products for this Gene ID 14939 View all products for this Gene ID 171528 View all products for this Gene ID
- UniProt
- View information about Granzyme B on UniProt.org
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