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ELISA MAX™ Standard Set Mouse IFN-γ

Pricing & Availability
Regulatory Status
RUO
Other Names
IFN-g ELISA Kit
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Product Citations
publications
mouse ifn-g_122109
  • mouse ifn-g_122109
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430801 5 plates 212€
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Mouse IFN-γ ELISA MAX™ Capture Antibody (200X)
  • Mouse IFN-γ ELISA MAX™ Detection Antibody (200X)
  • Mouse IFN-γ Standard
  • Avidin-HRP (1000X)

Product Details

Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Lau AH, et al. 2006. J. Leukocyte Biol. 79:941.
  2. Inoue Y, et al. 2007. J. Immunol. 179:764.
  3. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
  4. Tallima H, et. al. 2009. Cytokine 48:239. PubMed
  5. El Ridi R, et al. 2010. Microbes Infect. epub. PubMed
  6. He W, et al. 2010. J. Immunol. 185:126. PubMed
  7. Mori T, et al. 2014. J Immunol. 193:1728. PubMed
Product Citations
  1. Zuo J, et al. 2017. Mol Med Rep. 16:6946. PubMed
  2. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  3. Miranda K, et al. 2019. Front Immunol. 10:1049. PubMed
  4. Rother M, et al. 2018. Cell Host Microbe. 23:661. PubMed
  5. Uchimura T et al. 2018. Immunity. 49(6):1049-1061 . PubMed
  6. Westrich JA, et al. 2019. Oncogene. 38:7166. PubMed
  7. Fertan E, et al. 2019. Am J Pathol. 189:1435. PubMed
  8. Wilkinson R, et al. 2010. Nephrol Dial Transplant. 26:1483. PubMed
  9. He W, et al. 2010. J Immunol. 185:126. PubMed
  10. Mori T, et al. 2014. J Immunol. 193:1728. PubMed
  11. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  12. Elliott D, et al. 2016. J Pharmacol Exp Ther. 357: 177 - 187. PubMed
  13. Swee L, et al. 2016. Open Bio. 6: 160293. PubMed
  14. Giehl E, et al. 2021. Front Immunol. 610042:12. PubMed
  15. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
  16. Pandey S, et al. 2020. Cell Syst. 11:495. PubMed
  17. Zuo S, et al. 2021. EBioMedicine. 64:103240. PubMed
  18. Chetty A, et al. 2021. Cell Host Microbe. 29:579. PubMed
  19. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  20. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  21. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  22. Reeves KM, et al. 2021. Clin Cancer Res. Online ahead of print. PubMed
  23. Shibuya M, et al. 2021. iScience. 24:103131. PubMed
  24. Vijver SV, et al. 2021. Front Immunol. 12:733561. PubMed
  25. Zuo S, et al. 2021. J Immunother Cancer. 9:. PubMed
  26. Unsinger J, et al. 2021. J Leukoc Biol. 109:697. PubMed
  27. Kumar RK, et al. 2021. Vascul Pharmacol. 138:106838. PubMed
  28. Li L, et al. 2022. Stem Cell Res Ther. 13:479. PubMed
  29. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  30. Kang IH, et al. 2022. iScience. 25:104818. PubMed
  31. Hajjar S, et al. 2022. Cell Death Differ. 29:585. PubMed
  32. Watson F, et al. 2022. Am J Trop Med Hyg. . PubMed
  33. Saha S, et al. 2023. Sci Rep. 13:4609. PubMed
  34. Cho MJ, et al. 2023. Exp Mol Med. 55:1033. PubMed
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org
Go To Top Version: 2    Revision Date: 09.11.2013

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