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Pacific Blue™ anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Pricing & Availability
Clone
RB6-8C5 (See other available formats)
Regulatory Status
RUO
Other Names
Gr-1
Isotype
Rat IgG2b, κ
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Product Citations
publications
RB6-8C5_Blue_042909.jpg
C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PB (red histogram) or rat IgG2b, κ PB isotype control (green histogram).
  • RB6-8C5_Blue_042909.jpg
    C57BL/6 mouse bone marrow (gated on myeloid cell population) stained with Ly-6G/Ly-6C (clone RB6-8C5) PB (red histogram) or rat IgG2b, κ PB isotype control (green histogram).
See Pacific Blue™ spectral data
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108429 25 µg 76€
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108430 100 µg 172€
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Description

Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Raised against granulocytes of mouse origin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16,33-35, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
  2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
  3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
  4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
  5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
  6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
  8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
  9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
  10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
  11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
  12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
  13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
  14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
  15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
  16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
  17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
  18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
  19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
  23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
  24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
  25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
  26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
  28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
  29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
  30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
  31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
  32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
  33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
  34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
  35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)
Product Citations
  1. Manicone A, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.02.008. PubMed
  2. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  3. Gaya M et al. 2018. Cell. 172(3):517-533 . PubMed
  4. Schneider C, et al. 2018. Cell. 174:271. PubMed
  5. Arima Y et al. 2017. eLife. 6 pii: e25517. PubMed
  6. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  7. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  8. Taube C, et al. 2011. PLoS One. 6:e21799. PubMed
  9. Yan J, et al. 2013. Free Radic Biol Med. 23:5849. PubMed
  10. Hildebrand D, et al. 2013. J Immunol. 190:4812. PubMed
  11. Dow L, et al. 2014. PLoS One. 9:95236. PubMed
  12. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  13. Aguado B, et al. 2015. Sci Rep. 5: 17566. PubMed
  14. Rosen D, et al. 2016. J Infect Dis. 213: 649 - 658. PubMed
  15. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  16. Billroth-MacLurg, AC, et al. 2016. J Immunol. 197:2208-2218. PubMed
  17. Szafran BN, et al. 2021. Chemical Research in Toxicology. 34(6):1556-1571. PubMed
  18. Melo-Silva CR, et al. 2021. PLOS Pathogens. 17(5):e1009593. PubMed
  19. Manríquez V, et al. 2021. Nat Commun. 12:4547. PubMed
  20. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  21. Beauchamp EM, et al. 2021. Blood Cancer Discov. 6:. PubMed
  22. Chavez JS, et al. 2022. Cells. 11:. PubMed
  23. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  24. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  25. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  26. Dye BR, et al. 2020. Biomaterials. 234:119757. PubMed
  27. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  28. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  29. Kuwayama R, et al. 2022. Nat Commun. 13:3107. PubMed
  30. Hilfenhaus G, et al. 2021. Cancer Res. 81:619. PubMed
  31. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  32. Gupta S, et al. 2022. Sci Adv. 8:eabn4886. PubMed
  33. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  34. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  35. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  36. Melo-Silva CR, et al. 2022. Cell Rep. 41:111676. PubMed
  37. Li L, et al. 2023. iScience. 26:106095. PubMed
RRID
AB_893559 (BioLegend Cat. No. 108429)
AB_893556 (BioLegend Cat. No. 108430)

Antigen Details

Structure
21-25 kD
Distribution

Granulocytes, monocytes

Cell Type
Granulocytes, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID
17067 View all products for this Gene ID 546644 View all products for this Gene ID
UniProt
View information about Ly-6G Ly-6C on UniProt.org
Go To Top Version: 2    Revision Date: 12.09.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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