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PE anti-human CD13 Antibody

Pricing & Availability
Clone
WM15 (See other available formats)
Regulatory Status
RUO
Workshop
IV M44
Other Names
Aminopeptidase N, APN, gp150
Isotype
Mouse IgG1, κ
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Product Citations
publications
WM15_PE_061507
Human peripheral blood granulocytes stained with WM15 PE
  • WM15_PE_061507
    Human peripheral blood granulocytes stained with WM15 PE
See PE spectral data
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301703 25 tests 79€
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301704 100 tests 190€
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Description

CD13 is a 150-170 kD type II transmembrane glycoprotein also known as aminopeptidase N, APN, and gp150. This zinc metallopeptidase is expressed as a homodimer on granulocytes, myeloid progenitors, endothelial cells, epithelial cells and subset of granular lymphoid cells. It is not expressed on platelets or erythrocytes. CD13 is thought to be involved in the metabolism of many regulatory peptides and functions in antigen processing and the cleavage of chemokines such as MIP-1. CD13 serves as the cellular receptor for Coronavirus.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inhibition of tumor-cell invasion and blocking of aminopeptidase activities2,3, and immunohistochemical staining of acetone-fixed frozen tissue sections5. WM15 does not recognize formalin-fixed or paraffin-embedded tissue sections5. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301708). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301723 and 301724) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Saiki I, et al. 1993. Int J Cancer. 54:137. (Block)
  3. Rosenzwajg M, et al. 2000. Blood 95:453. (Block)
  4. Kawase M, et al. 2008. J Virol. 83:712. (Block) PubMed
  5. Di Matteo P, et al. 2011. J. Histochem. Cytochem. 59:47. (IHC)
Product Citations
  1. Bombelli S,et al. 2017. Am J Pathol. . 10.1016/j.ajpath.2017.09.012. PubMed
  2. Wu L, et al. 2018. Oncol Lett. 15:9507. PubMed
  3. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  4. Zeng Y et al. 2019. Immunity. 51(5):930-948 . PubMed
  5. Man L, et al. 2020. Cell Reports. 32(6):108027. PubMed
  6. Boxx G, et al. 2010. Infect Immun. 78:1250. PubMed
  7. Valentijn A, et al. 2013. Hum Reprod. 28:2695. PubMed
  8. Siciliano C, et al. 2016. Stem Cell Res. 17: 342-351. PubMed
  9. Zhang H, et al. 2017. Oncogene. 10.1038/onc.2016.512. PubMed
  10. Mpakou V, et al. 2021. Exp Ther Med. 1.010416667. PubMed
  11. Zhao M, et al. 2021. Frontiers in Cell and Developmental Biology. 9:652972. PubMed
  12. Qu M, et al. 2021. Frontiers in Oncology. 11:659720. PubMed
  13. Melzer C, et al. 2020. Int J Mol Sci. :21. PubMed
  14. Xin Y, et al. 2021. Stem Cell Res Ther. 49:12. PubMed
  15. Smith-Díaz CC, et al. 2021. Front Oncol. 11:709543. PubMed
  16. Yao J, et al. 2022. Front Pharmacol. 13:876076. PubMed
  17. Qi J, et al. 2022. Nat Commun. 13:1742. PubMed
  18. Gill D, et al. 2022. Elife. 11:. PubMed
  19. Ma X, et al. 2022. Front Oncol. 12:855570. PubMed
  20. Li F, et al. 2022. Front Pharmacol. 13:1001552. PubMed
  21. Zhao M, et al. 2022. J Oncol. 2022:3345536. PubMed
  22. Robb KP, et al. 2022. Front Immunol. 13:972095. PubMed
  23. Guahmich NL, et al. 2023. Commun Biol. 6:7. PubMed
  24. Xu Y, et al. 2022. Nat Genet. 54:1690. PubMed
RRID
AB_314179 (BioLegend Cat. No. 301703)
AB_314180 (BioLegend Cat. No. 301704)

Antigen Details

Structure
Zinc metallopeptidase, type II integral membrane glycoprotein, 150-170 kD
Distribution

Granulocytes, monocytes, myeloid progenitors, endothelial and epithelial cells, granular lymphocyte subset

Function
Zinc-binding metalloproteinase, antigen processing, cleaves MIP-1 chemokine
Ligand/Receptor
Coronavirus receptor
Cell Type
Endothelial cells, Epithelial cells, Granulocytes, Hematopoietic stem and progenitors, Lymphocytes, Mesenchymal Stem Cells, Monocytes, Neutrophils
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Shipp M, et al. 1993. Blood 82:1052.
2. Larsen S, et al. 1996. J. Exp. Med. 184:183.

Gene ID
290 View all products for this Gene ID
UniProt
View information about CD13 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 03.21.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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