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PE anti-human CD154 Antibody

Pricing & Availability
Clone
24-31 (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5
Isotype
Mouse IgG1, κ
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Product Citations
publications
24-31
PMA+ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 PE
  • 24-31
    PMA+ionomycin-stimulated human PBMCs (6 hours) stained with 24-31 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
310805 25 tests 105€
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310806 100 tests 211€
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Description

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1,3 and blocking of T cell-dependent B cell differentiation1,2,4,5. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 310812). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 310828) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Brams P, et al. 2001. Int. Immunopharmacol. 1:277. (Block, IF)
  2. Rushworth SA, et al. 2002. Transplantation 73:635. (Block)
  3. Berner B, et al. 2000. Ann. Rheum. Dis. 59:190. (IF)
  4. Nordström T, et al. 2006. J. Leukocyte Biol. 79:319. (Block)
  5. Zhang AL, et al.2007. Blood doi:10.1182/blood-2007-02-076364. (Block) PubMed
  6. Kuchen S, et al. 2007. J. Immunol. 179:5886.
  7. Matus-Nicodermos R, et al. 2011. J. Immunol. 186:2164. PubMed
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed
  2. Yang W, et al. 2019. Nat Med. 25:767. PubMed
  3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  4. Kuchen S, et al. 2007. J Immunol. 179:5886. PubMed
  5. Karnell J, et al. 2011. J Immunol. 187:3603. PubMed
  6. Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
  7. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  8. Meckiff BJ, et al. 2020. Cell. 183(5):1340-1353.e16. PubMed
  9. Bziat V, et al. 2021. Cell. . PubMed
  10. Zhang J, et al. 2020. Nat Microbiol. . PubMed
  11. Biondi BE, et al. 2021. Contemp Clin Trials Commun. 21:100704. PubMed
  12. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  13. Yu ED, et al. 2022. Cell Rep Methods. 2:100350. PubMed
  14. Lim JME 2023. STAR Protocols. 4(1):101995. PubMed
  15. Pandit H, et al. 2023. iScience. 26:105929. PubMed
  16. Babl N, et al. 2023. Front Oncol. 13:1107484. PubMed
  17. Collora JA, et al. 2022. Immunity. 55:1013. PubMed
  18. Veatch JR, et al. 2022. Cancer Cell. 40:393. PubMed
  19. Wang Y, et al. 2023. Hum Vaccin Immunother. 19:2202127. PubMed
RRID
AB_314828 (BioLegend Cat. No. 310805)
AB_314829 (BioLegend Cat. No. 310806)

Antigen Details

Structure
TNF superfamily, type II transmembrane glycoprotein, cleaved as soluble CD40L, 32-39 kD
Distribution

Activated T cells

Function
B cell costimulation
Ligand/Receptor
CD40
Cell Type
T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Najafian N, et al. 2003. Expert Opin. Biol. Ther. 3:227.
2. Racke M, et al. 2002. Expert Opin. Ther. Targets. 6:275.
3. Ford G, et al. 1999. J. Immunol. 162:4037.

Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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