- Clone
- 2HUB-159 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- VI C-111
- Other Names
- IFN-γRβ, IFNγAF-1
- Isotype
- Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2HUB-159_PE_072909.jpg 2HUB-159_PE_072909.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2HUB-159_PE_072909.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human IFN-γ Rβ transfected L929 cells stained with 2HUB-159 PE
Cat # | Size | Price | Quantity Check Availability | Save | ||
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308504 | 100 tests | 244€ |
IFN-γ R β chain is a 65 kD type I transmembrane protein, also known as accessory factor (AF-1) or IFN-γRII. IFN-γ receptor is composed of an IFN-γ R α chain (CDw119) and a β chain. They are members of the class II cytokine receptor family. The α chain is required for ligand binding and ligand trafficking to the cells and also plays some role in signal transduction. The β chain is critical for delivering IFN-γ mediated pleiotropic signals. IFN-γ R β chain is broadly expressed on a variety of cells at low levels and upregulated on some activated B cells.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Hamster
- Immunogen
- soluable extracellular domains of the human IFN-γ R β chain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Additional reported applications (for the relevant formats) include: Western blotting2,3 and immunoprecipitation2,3. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 308504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)). TThe LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308506).
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Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
- Bach EA, et al. 1996. Mol. Cell. Biol. 16:3214. (IP WB)
- Rosenzweig SD, et al. 2004. J. Immunol. 173:4000. (IP WB)
- Joseph TD, et al. 2001. J. Biol. Chem. 276:47136.
- Choi JC, et al. 2007. J. Immunol. 178:1598.
- Tjon AS, et al. 2014. J Immunol. 192:5625. PubMed
- Product Citations
- RRID
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AB_314718 (BioLegend Cat. No. 308504)
Antigen Details
- Structure
- Ig superfamily, class II cytokine receptor family, associates with CDw119, 65 kD
- Distribution
-
All cells, except erythrocytes, at low levels
- Function
- Transducing signals
- Ligand/Receptor
- IFN-γ
- Biology Area
- Immunology
- Molecular Family
- Cytokine/Chemokine Receptors
- Antigen References
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1. Basler C, et al. 2002. Int. Rev. Immunol. 21:305.
2. Brierley M, et al. 2002. J. Interferon Cytokine Res. 22:835. - Gene ID
- 3459 View all products for this Gene ID
- UniProt
- View information about IFN-gamma R beta chain on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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