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PE/Cyanine7 anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Regulatory Status
RUO
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PECy7_061807
Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine7
  • HIB19_PECy7_061807
    Human peripheral blood lymphocytes stained with HIB19 PE/Cyanine7
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
302215 25 tests 115€
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302216 100 tests 234€
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Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

 

Clone HIB19 partially blocks anti-human CD19 clones 4G7 and SJ25C1 staining based on in-house testing

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
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  2. Hao Y, et al. 2018. Exp Ther Med. 15:1403. PubMed
  3. Tardif V, et al. 2019. Nat Commun. 10:823. PubMed
  4. Roy Chowdhury R, et al. 2018. Nature. 560:644. PubMed
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  8. Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
  9. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
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  13. Watanabe A, et al. 2019. Cell. 177:1124. PubMed
  14. Cillo AR, et al. 2020. Immunity. 52(1):183-199.e9.. PubMed
  15. Napolitano R, et al. 2020. Oncol Rep. 44:1561. PubMed
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  18. Lindner S, et al. 2013. Cancer Res. 73:2468. PubMed
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  23. Joosten S, et al. 2016. PLoS Pathog. 12: 1005687. PubMed
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  27. Gajdasik DW, et al. 2020. Nat Commun. 2.834027778. PubMed
  28. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  29. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  30. Dugan HL, et al. 2021. Immunity. 54(6):1290-1303.e7. PubMed
  31. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  32. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
  33. Pereira JL, et al. 2021. Transl Oncol. 14:101125. PubMed
  34. van Heerden D, et al. 2021. Int J Mol Sci. 22:. PubMed
  35. Martín Monreal MT, et al. 2021. Front Immunol. 12:716250. PubMed
  36. Li X, et al. 2021. Front Immunol. 12:779560. PubMed
  37. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  38. Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed
  39. Zou J, et al. 2022. J Clin Invest. 132:. PubMed
  40. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  41. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  42. Li Y, et al. 2021. Nat Commun. 12:87. PubMed
  43. Kornauth C, et al. 2021. Haematologica. 106:2251. PubMed
  44. Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
  45. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  46. Li C, et al. 2022. J Exp Med. 219:. PubMed
  47. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  48. Connors J, et al. 2022. Geroscience. :. PubMed
  49. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  50. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  51. Masuda T, et al. 2022. iScience. 25:104781. PubMed
  52. Szklarski M, et al. 2021. Front Immunol. 12:644548. PubMed
  53. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  54. SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed
  55. Wildner NH, et al. 2021. J Leukoc Biol. 109:77. PubMed
  56. Liu L, et al. 2022. Sci Transl Med. 14:eabn6859. PubMed
  57. Omer-Javed A, et al. 2022. Cell. 185:2248. PubMed
  58. Tessaro FHG, et al. 2022. Cell Rep. 39:110977. PubMed
  59. Vazquez-Lombardi R, et al. 2022. Immunity. 55:1953. PubMed
  60. Voit RA, et al. 2023. Nat Immunol. 24:69. PubMed
  61. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  62. García-García A, et al. 2023. Bioact Mater. 24:174. PubMed
  63. Alkashgari HR, et al. 2022. Int J Mol Sci. 24: . PubMed
  64. Han CY, et al. 2022. Redox Biol. 51:102263. PubMed
  65. Mittler E, et al. 2022. Sci Transl Med. 14:eabl5399. PubMed
  66. Parasar P, et al. 2022. Am J Reprod Immunol. 88:e13614. PubMed
  67. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
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RRID
AB_314245 (BioLegend Cat. No. 302215)
AB_314246 (BioLegend Cat. No. 302216)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org
Go To Top Version: 2    Revision Date: 12.18.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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