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PE/Cyanine7 anti-human TIGIT (VSTM3) Antibody

Pricing & Availability
Clone
A15153G (See other available formats)
Regulatory Status
RUO
Other Names
T-cell immunoreceptor with Ig and ITIM domains, VSIG9, VSTM3, WUCAM
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
A15153G_PECyanine7_TIGIT_Antibody_1_110823
Human peripheral blood leukocytes were stained with FITC CD3 and TIGIT (clone A15153G) PE/Cyanine7 (left) or PE/Cyanine7 mouse IgG2a, κ PE/Cyanine7 isotype control (right). Data shown was gated on the lymphocytes population.
  • A15153G_PECyanine7_TIGIT_Antibody_1_110823
    Human peripheral blood leukocytes were stained with FITC CD3 and TIGIT (clone A15153G) PE/Cyanine7 (left) or PE/Cyanine7 mouse IgG2a, κ PE/Cyanine7 isotype control (right). Data shown was gated on the lymphocytes population.
See PE/Cyanine7 spectral data
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372713 25 tests 147€
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372714 100 tests 344€
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Description

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3 or WUCAM, is a 26 kD, type I transmembrane protein and is a member of the PVR (poliovirus receptor) family of immunoglobulin-like domain containing proteins. TIGIT is expressed on activated T cells, follicular T helper, memory, and regulatory T cells as well as on NK cells. TIGIT is a negative regulator of NK and T cell activation. Expression of TIGIT is associated with decreased functionality of CD8 T cells in chronic viral infection and tumors. TIGIT also promotes the differentiation of tolerogenic phenotype in dendritic cells with an increased secretion of IL-10 and a diminished production of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human TIGIT.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This clone can suppress anti-CD3 induced T cell proliferation in vitro based on in-house testing.

This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P).

Additional reported applications (for the relevant formats) include: Blocking1.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Stamm H, et al. 2018. Oncogene. Pubmed
Product Citations
  1. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  2. Qi J, et al. 2021. Cell Reports Medicine. 2(8):100353. PubMed
  3. Cheng J, et al. 2022. iScience. 25:103588. PubMed
  4. Zhang X, et al. 2021. Front Immunol. 12:602492. PubMed
  5. Li P, et al. 2022. Front Oncol. 12:917400. PubMed
  6. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  7. Herter JM, et al. 2022. Strahlenther Onkol. Online ahead of print. PubMed
  8. Wang CQ, et al. 2023. Cells. 12: . PubMed
  9. Chen M, et al. 2023. Cell Biosci. 13:19. PubMed
  10. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  11. Hu Y, et al. 2022. Cell Res. 995:32. PubMed
RRID
AB_2632928 (BioLegend Cat. No. 372713)
AB_2632929 (BioLegend Cat. No. 372714)

Antigen Details

Structure
26kD; type I transmembrane protein, Ig-like V-type domain, ITIM motif.
Distribution

Activated T cells, Regulatory T cells (Treg), Follicular Helper T cells (TFH), NK cells.

Function
Cell signaling, negative regulation of T cells, T cell tolerance, T cell anergy.
Ligand/Receptor
CD155 (PVR), CD112 (PVRL2, NECTIN-2).
Cell Type
NK cells, T cells, Tfh, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Inhibitory Molecules, Signal Transduction
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Stanietsky N, et al. 2009. Proc. Natl. Acad. Sci. 106:17858.
2. Yu X, et al. 2009. Nat. Immunol. 10:48.
3. Johnston R, et al. 2014. Cancer Cell. 26:923.

Gene ID
201633 View all products for this Gene ID
UniProt
View information about TIGIT on UniProt.org
Go To Top Version: 1    Revision Date: 01.02.2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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