Loading...

PE/Dazzle™ 594 anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
EH12.2H7 (See other available formats)
Regulatory Status
RUO
Other Names
PD-1, PDCD1
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
EH12.2H7_PEDazzle594_CD297_Antibody_FC_1_060914
Human peripheral blood lymphocytes were stained with CD3 APC and CD279 (clone EH12.2H7) PE/Dazzle™ 594 (above) or mouse IgG1 PE/Dazzle™ 594 isotype control (below).
  • EH12.2H7_PEDazzle594_CD297_Antibody_FC_1_060914
    Human peripheral blood lymphocytes were stained with CD3 APC and CD279 (clone EH12.2H7) PE/Dazzle™ 594 (above) or mouse IgG1 PE/Dazzle™ 594 isotype control (below).
  • EH12.2H7_PEDazzle594_CD297_Antibody_FC_2_060914
  • EH12.2H7_PEDazzle594_CD297_Antibody_FC_3_060914
    PHA-stimulated (day 3) human peripheral blood lymphocytes stained with CD279 (clone EH12.2H7) PE/Dazzle™ 594 (filled histogram) or mouse IgG1 PE/Dazzle™ 594 (open histogram).
See PE/Dazzle™ 594 spectral data
Cat # Size Price Quantity Check Availability Save
329939 25 tests 128€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
329940 100 tests 315€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Dazzle™ 594 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PE/Dazzle™ 594 has a maximum excitation of 566 nm and a maximum emission of 610 nm.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections13, and spatial biology (IBEX)15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
  2. Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
  3. Velu V, et al. 2007. J. Virol. 81:5819. (FA)
  4. Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
  5. Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
  6. Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
  7. Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
  8. Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
  9. Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
  10. Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
  11. Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
  12. Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
  13. Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
  14. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  15. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  16. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Taylor HE, et al. 2018. AIDS. 32:2847. PubMed
  2. Toor SM, et al. 2018. Clin Exp Immunol. 191:189. PubMed
  3. Valpione S, et al. 2020. Nat Cancer. 0.1875. PubMed
  4. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  5. Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed
  6. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  7. Naik A, et al. 2021. J Cell Mol Med. 25:10376. PubMed
  8. Cordero H, et al. 2021. Nat Commun. 12:5761. PubMed
  9. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  10. Tarke A, et al. 2022. Cell. 185:847. PubMed
  11. NULL, et al. 2022. Cell. 185:916. PubMed
  12. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
  13. Sun P, et al. 2022. iScience. 25:105202. PubMed
RRID
AB_2563658 (BioLegend Cat. No. 329939)
AB_2563659 (BioLegend Cat. No. 329940)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Transiently expressed on CD4- CD8- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells

Ligand/Receptor
B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org
Go To Top Version: 2    Revision Date: 01.22.2015

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account