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PerCP anti-human CD4 Antibody

Pricing & Availability
Clone
RPA-T4 (See other available formats)
Regulatory Status
RUO
Workshop
IV T114
Other Names
T4
Isotype
Mouse IgG1, κ
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Product Citations
publications
RPA-T4_PerCP_020608
Human peripheral blood lymphocytes stained with RPA-T4 PerCP
  • RPA-T4_PerCP_020608
    Human peripheral blood lymphocytes stained with RPA-T4 PerCP
See PerCP spectral data
Cat # Size Price Quantity Check Availability Save
300527 25 tests 123€
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300528 100 tests 268€
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD4 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11.  This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
  2. Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
  3. Deng MC, et al. 1995. Circulation 91:1647. (IHC)
  4. Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
  5. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  6. Lan RY, et al. 2006. Hepatology 43:729.
  7. Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Boehm D et al. 2017. Cell host & microbe. 21(5):569-579 . PubMed
  2. Venugopal G, et al. 2017. PLoS One. . 10.1371/journal.pone.0188138. PubMed
  3. Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed
  4. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  5. Tran TM, et al. 2020. Immunity. 51(4):750-765. PubMed
  6. Smolders J, et al. 2010. PLoS One. 5:e15235. PubMed
  7. Bikker A, et al. 2012. Ann Rheum Dis. 71:1027. PubMed
  8. Conti L, et al. 2014. J Immunol. 188:1011. . PubMed
  9. Boyle M, et al. 2015. J Infect Dis. 212: 416-425. PubMed
  10. Petit N, et al. 2015. PLoS One. 10: 0138420. PubMed
  11. Nakaya H, et al. 2016. Proc Natl Acad Sci U S A. 113: 1853 - 1858. PubMed
  12. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  13. Ye H, et al. 2016. Exp Mol Pathol. 101:341-345. PubMed
  14. Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed
  15. Diao B, et al. 2020. Front Immunol. 1.032638889. PubMed
  16. Vallejo-Gracia A, et al. 2020. Nat Microbiol. 1.002777778. PubMed
  17. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  18. Rajashekar JK, et al. 2021. Cell Host Microbe. 29(6):904-916.e6. PubMed
  19. Zhao J, et al. 2021. Front Immunol. 12:658420. PubMed
  20. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  21. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  22. Cherneha M, et al. 2021. Viruses. 13:. PubMed
  23. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  24. De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed
  25. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
RRID
AB_893327 (BioLegend Cat. No. 300527)
AB_893321 (BioLegend Cat. No. 300528)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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