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PerCP/Cyanine5.5 anti-human CD8 Antibody

Pricing & Availability
Clone
SK1 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
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Product Citations
publications
SK1_PerCPCyanine55_CD8_Antibody_030519_updated.png
Human peripheral blood lymphocytes stained with CD8 (clone SK1) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
  • SK1_PerCPCyanine55_CD8_Antibody_030519_updated.png
    Human peripheral blood lymphocytes stained with CD8 (clone SK1) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
344709 25 tests 141€
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344710 100 tests 292€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone SK1 recognizes the a chain of CD8. Additional reported applications (for the relevant formats) include: proteogenomics8, immunohistochemistry of acetone-fixed frozen tissue sections, and spatial biology (IBEX)9,10. This clone was tested in-house and does not demonstrate utility for formalin-fixed paraffin-embedded (FFPE) human tonsil sections.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310.
  2. Campanelli R, et al. 2002. Intl. Immunol. 14:39.
  3. Evans RL, et al. 1981. Immunol. 78:544.
  4. Wooldridge L, et al. 2005. J. Bio. Chem. 280:27491.
  5. Ch'el IL, et al. 2011. J Exp Med. 208:633. PubMed
  6. Carbone A, et al. 1999. Blood 93:2319. (IHC-F)
  7. Ahmed A, et al. 2001. J. Pathol. 193:383. (IHC)
  8. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Terzioglu G,et al. 2017. Mech Ageing Dev. . 10.1016/j.mad.2017.09.003. PubMed
  2. Khamaikawin W, et al. 2018. Mol Ther Methods Clin Dev. 9:23. PubMed
  3. Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed
  4. Minagawa A, et al. 2018. Cell Stem Cell. 1.548611111. PubMed
  5. Shehata L, et al. 2019. Nat Commun. 10:1126. PubMed
  6. Peng S et al. 2019. Cell Rep. 28(10):2728-2738 . PubMed
  7. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  8. Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
  9. Ringpis G, et al. 2012. PLoS One. 7:e53492. PubMed
  10. Koelsch K, et al. 2013. PLoS One. 8:50068. PubMed
  11. Fang L, et al. 2013. Neuro Oncology. 15:1479. PubMed
  12. Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
  13. Søgaard O, et al. 2015. PLoS One. 11: 1005142. PubMed
  14. Kim D, et al. 2020. Nat Med. 26:236. PubMed
  15. Mineo M, et al. 2020. Molecular Cell. 78(6):1207-1223.e8. PubMed
  16. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  17. Wong SS, et al. 2021. Cell Reports Medicine. 2(4):100237. PubMed
  18. Fels JM, et al. 2021. Cell. 184(13):3486-3501.e21. PubMed
  19. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  20. Basar R, et al. 2021. Cell Reports. 36(3):109432. PubMed
  21. Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
  22. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  23. Basar R, et al. 2020. bioRxiv. . PubMed
  24. Sakharkar M, et al. 2021. Sci Immunol. :6. PubMed
  25. Parenti S, et al. 2021. NPJ Precis Oncol. 5:4. PubMed
  26. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  27. Lin G, et al. 2021. Mol Med Rep. 23:. PubMed
  28. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  29. Wang X, et al. 2021. Adv Sci (Weinh). 8:e2101447. PubMed
  30. Arakawa A, et al. 2021. J Immunol. 207:2235. PubMed
  31. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  32. Rappazzo CG, et al. 2022. Immunity. 55:1710. PubMed
  33. García-Ríos E, et al. 2022. Biomedicines. 10:. PubMed
  34. Zhang J, et al. 2022. iScience. 25:105340. PubMed
  35. Hagel J, et al. 2021. J Immunol. 206:3073. PubMed
  36. Chow A 2023. Immunity. 56(1):93-106.e6. PubMed
  37. Gfeller D, et al. 2023. Cell Syst. 14:72. PubMed
  38. Wang P, et al. 2023. Front Immunol. 13:1104329. PubMed
  39. Statzu M, et al. 2023. Nat Microbiol. 8:299. PubMed
  40. Wu B, et al. 2023. J Immunother Cancer. 11: . PubMed
  41. Mittler E, et al. 2022. Sci Transl Med. 14:eabl5399. PubMed
  42. Woodcock V, et al. 2023. BJUI Compass. 4:322. PubMed
  43. Li N, et al. 2023. Nat Commun. 14:1986. PubMed
  44. Chernyshev M, et al. 2023. Nat Commun. 14:2249. PubMed
  45. Kaku CI, et al. 2023. Nat Commun. 14:2751. PubMed
RRID
AB_2044009 (BioLegend Cat. No. 344709)
AB_2044010 (BioLegend Cat. No. 344710)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8b, 32-34 kD
Distribution

Majority of thymocytes, T cell subset, NK cells

Function
MHC class I co-receptor, thymic differentiation, T cell activation
Ligand/Receptor
MHC Class I molecules
Cell Type
NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.

Gene ID
925 View all products for this Gene ID
UniProt
View information about CD8 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 8    Revision Date: 03.05.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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