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Purified anti-β-actin Antibody

Pricing & Availability
Clone
W16197A (See other available formats)
Regulatory Status
RUO
Other Names
ACTB, Actin, cytoplasmic 1, Beta-actin
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16197A_PURE_beta-actin_Anitbody_1_101217
Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_1_101217
    Total lysates (15 µg protein) from HeLa, NIH3T3, rat brain and zebra fish were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:2000 diluted (0.1 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_2_101217
    Total lysates (15 µg protein) from HeLa was resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:800 diluted (0.25 µg/mL), 1:1600 diluted (0.125 µg/mL), 1:3200 diluted (0.0625 µg/mL), 1:6400 diluted (0.03125 µg/mL) and 1:12800 diluted (0.0155 µg/mL) purified anti-β-actin antibody (upper). Proteins were visualized using an HRP anti rat-IgG secondary antibody (cat# 405405) or HRP Donkey anti-rabbit IgG Antibody (cat# 406401) for GAPDH and chemiluminescence detection. GAPDH (poly6314, cat# 631401) antibody was used as a loading control (lower). Lane M: MW ladder.
  • W16197A_PURE_beta-actin_Anitbody_3_081619
    Western blot of purified anti-β-actin antibody (clone W16197A). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
664801 25 µg 81€
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664802 100 µg 203€
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Description

β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human,Mouse,Rat,Zebrafish,Drosophila
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human ACTB peptide (1-13 a.a.).
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg/ml in human, mouse, rat and zebrafish and 0.1 - 0.5 µg/ml in Drosophila. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only verified with the purified format.

Product Citations
  1. Balcioglu O, et al. 2020. Breast Cancer Res. 1.003472222. PubMed
  2. Lippert TP, et al. 2021. Nat Commun. 0.855555556. PubMed
  3. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  4. Ren Y, et al. 2022. J Immunother Cancer. 10:. PubMed
  5. Wang L, et al. 2022. Viruses. 14: . PubMed
  6. Park SJ, et al. 2023. Int J Mol Sci. 24: . PubMed
RRID
AB_2721350 (BioLegend Cat. No. 664801)
AB_2721349 (BioLegend Cat. No. 664802)

Antigen Details

Structure
375 amino acids with a predicted molecular weight of 42 kD.
Distribution

Cytoplasm

Function
Actins are highly conserved proteins that are involved in cell motility.
Interaction
Interacts with CPNE1 and CPNE4, ERBB2, GCSAM, TBC1D21.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Microfilaments
Antigen References

1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673.
2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133.
3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.

Gene ID
60 View all products for this Gene ID
UniProt
View information about beta-actin on UniProt.org
Go To Top Version: 2    Revision Date: 08.16.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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