- Clone
- Poly18020 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, Tubulin beta-3 chain, tubulin beta-III, TUBB3, tubulin beta-4 chain, class III beta-tubulin
- Previously
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Covance Catalog# PRB-435P
- Isotype
- Rabbit Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918 A_Poly18020_PURE_Tubulin_beta3_IHCP_1_032918](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly18020_PURE_Tubulin_beta3_IHCP_1_032918.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded rat cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020) on formalin-fixed paraffin-embedded human cerebellum. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Western blot of purified anti-Tubulin β-3 (TUBB3) antibody (Poly18020). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blots were incubated with 0.1 µg/mL of Poly18020 or Rabbit IgG overnight at 4°C, followed by incubation with HRP-labeled anti-rabbit IgG (Cat. No. 410406). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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802001 | 100 µL | 347€ |
Tubulin is the main component of microtubules. In adults, tubulin beta 3 (TUBB3) is primarily expressed in neurons and is commonly used as a neuronal marker. It plays an important role in neuronal cell proliferation and differentiation. Mutations in this gene cause congenital fibrosis of the type 3 extraocular muscles. Tubulin beta 3 (TUBB3) is also found in a wide range of tumors. Studies indicate that it is a predictive and prognostic marker in various tumors.
Product DetailsProduct Details
- Reactivity
- Human,Mouse,Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- This antibody was raised against microtubules derived from rat brain.
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
WB - Verified
ICC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.1 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunocytochemistry (ICC).
This antibody is well characterized and highly reactive to neuron specific Class III ß-Tubulin (ßIII), but does not identify ß-tubulin found in glial cells. This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Feuer R, et al. 2005. J. Neurosci. 25(9):2434-44.
- Mozzetti S, et al. 2005. Clin. Cancer Res. 11(1):298-305.
- Feuer R, et al. 2003. Am. J. Pathol. 163(4):1379-93.
- Zhang XM, Yang XJ. 2001. Development. 128(6):943-57.
- Ma M, et al. 2012. Neurobiol Dis. 56:34. (WB) PubMed
- Tseung G, et al. 2011. J. Virol. 85:5718. (IF) PubMed
- Shimada IS, et al. 2012. J Neurosci. 32:7926. (IHC) PubMed
- Flores-Otero J, et al. 2007. J. Neurosci. 27:14023. (IF) PubMed
- Product Citations
- RRID
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AB_2564645 (BioLegend Cat. No. 802001)
Antigen Details
- Cell Type
- Mature Neurons, Neural Stem Cells
- Biology Area
- Cell Biology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Microtubules
- Gene ID
- 10381 View all products for this Gene ID
- UniProt
- View information about Tubulin beta-3 on UniProt.org
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