Ultra-LEAF™ Purified anti-mouse CD3 Antibody

Product Details

Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

γδTCR-positive T-T hybridoma D1

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
IHC-F, IP, ICC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume or 100 µl of whole blood.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)^1,2.

Application References

(PubMed link indicates BioLegend citation)

1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

1. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
2. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
3. Radulovic V, et al. 2020. Cell Reports. 27(10):2826-2836.e5.. PubMed
4. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
5. Fajstova A, et al. 2020. Cells. 9:00. PubMed
6. De Cicco P, et al. 2019. British Journal of Pharmacology. 177(4):884-897. PubMed
7. Wang J, et al. 2021. Am J Cancer Res. 11:2005. PubMed
8. Saito S, et al. 2020. Nutrients. 12:. PubMed
9. Watson MJ, et al. 2021. Nature. 591:645. PubMed
10. Gkountidi AO, et al. 2021. Cancer Immunol Res. 9:748. PubMed
11. Anastasiou M, et al. 2021. JCI Insight. 6:. PubMed
12. Teh MR, et al. 2021. Front Immunol. 12:714613. PubMed
13. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
14. Xu L, et al. 2022. Nat Commun. 13:6881. PubMed
15. Ocaña-Guzman R, et al. 2022. Cells. 11: . PubMed
16. Klaus T, et al. 2022. JCI Insight. 7: . PubMed
17. Jiang YP, et al. 2022. Int J Mol Sci. 24: . PubMed
18. Shen M, et al. 2022. Adv Sci (Weinh). 9:e2203523. PubMed

RRID

AB_2810317 (BioLegend Cat. No. 100256)
AB_2810316 (BioLegend Cat. No. 100255)
AB_2810315 (BioLegend Cat. No. 100254)
AB_2810313 (BioLegend Cat. No. 100239)
AB_2561487 (BioLegend Cat. No. 100238)
AB_2810314 (BioLegend Cat. No. 100253)

Antigen Details

Structure

Ig superfamily, CD3/TCR, 20 kD

Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function

Antigen recognition, TCR signal transduction, T cell activation

Ligand/Receptor

Peptide antigen/MHC-complex

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID

12502 View all products for this Gene ID

UniProt

View information about CD3 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis