- Clone
- RPA-T4 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV T114
- Other Names
- T4
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RPA-T4_BV421_1_032911 RPA-T4_BV421_1_032911](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RPA-T4_BV421_1_032911.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD3 FITC and CD4 (clone RPA-T4) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom). -
Human peripheral mononuclear cells were fixed with 1% Paraformaldehyde (PFA), and then stained with 5 µg/ml of CD4 (clone RPA-T4) Brilliant Violet 421™ for 30 minutes at room temperature. The image was captured by 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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300531 | 25 tests | 152€ | ||||
300532 | 100 tests | 305€ |
CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules, and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
ICC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
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The RPA-T4 antibody binds to the D1 domain of CD4 (CDR1 and CDR3 epitopes) and can block HIV gp120 binding and inhibit syncytia formation. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections3,4,5, blocking of T cell activation1,2, and spatial biology (IBEX)10,11. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300569 - 300574).
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York. (Activ)
- Moir S, et al. 1999. J. Virol. 73:7972. (Activ)
- Deng MC, et al. 1995. Circulation 91:1647. (IHC)
- Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
- Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
- Lan RY, et al. 2006. Hepatology 43:729.
- Zenaro E, et al. 2009. J. Leukoc. Biol. 86:1393. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
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- RRID
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AB_10900084 (BioLegend Cat. No. 300531)
AB_10965645 (BioLegend Cat. No. 300532)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 55 kD
- Distribution
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T cell subset, majority of thymocytes, monocytes/macrophages
- Function
- MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
- Ligand/Receptor
- MHC class II molecules, HIV gp120, IL-16
- Cell Type
- Dendritic cells, Macrophages, Monocytes, T cells, Thymocytes, Tregs
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723. - Gene ID
- 920 View all products for this Gene ID
- UniProt
- View information about CD4 on UniProt.org
Related FAQs
- I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
- TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
- What is the F/P ratio range of our BV421™ format antibody reagents?
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It is lot-specific. On average it ranges between 2-4.
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD4
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Biotin anti-human CD4
Human peripheral blood lymphocytes stained with biotinylated... -
FITC anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 FITC Confocal image of human lymph node sample acquired using the... -
PE anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE Human peripheral blood was stained with CD4 (clone RPA-T4) P... -
PE/Cyanine5 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy... -
PE/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PE/Cy... -
Purified anti-human CD4
Human peripheral blood lymphocytes stained with purified RPA... -
APC/Cyanine7 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Alexa Fluor® 488 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa... Human peripheral blood mononuclear cells were fixed with 2% ... Human peripheral blood mononuclear cells were fixed with 1% ... -
Alexa Fluor® 647 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa... Confocal image of human spleen sample acquired using the IBE... Confocal image of human lymph node sample acquired using the... -
Pacific Blue™ anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Pacif... -
Brilliant Violet 421™ anti-human CD4
Human peripheral mononuclear cells were fixed with 1% Parafo... Human peripheral blood lymphocytes were stained with CD3 FIT... -
Alexa Fluor® 700 anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 Alexa... Confocal image of human metastatic lymph node sample acquire... -
PerCP anti-human CD4
Human peripheral blood lymphocytes stained with RPA-T4 PerCP -
PerCP/Cyanine5.5 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 570™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 FIT... -
Brilliant Violet 650™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Purified anti-human CD4 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 145Nd-an... -
Alexa Fluor® 594 anti-human CD4
Human peripheral blood mononuclear cells were fixed with 2% ... Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 510™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
PE/Dazzle™ 594 anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 785™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 605™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
Brilliant Violet 711™ anti-human CD4
Human peripheral blood lymphocytes were stained with CD4 (cl... -
APC/Fire™ 750 anti-human CD4
Human peripheral blood lymphocytes were stained with CD3 FIT... -
CD4 Fluorophore Sampler Kit
Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... -
CD4 Fluorophore Sampler Kit with Veri-Cells™ PBMC
Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... Veri-Cells™ PBMC were stained with anti-human CD4 (clone RPA... -
TotalSeq™-A0072 anti-human CD4
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TotalSeq™-B0072 anti-human CD4
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TotalSeq™-C0072 anti-human CD4
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Ultra-LEAF™ Purified anti-human CD4
Human peripheral blood lymphocytes stained with LEAF™ purifi... -
TotalSeq™-D0072 anti-human CD4
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