- Clone
- PC10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Proliferating Cell Nuclear Antigen, DNA Polymerase δ Auxiliary Protein
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a.PCNA a.PCNA](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/PCNA.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Hela cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-PCNA antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system. -
HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then the cells were intracellularly stained with 5 µg/ml of purified anti-human/mouse/rat PCNA (clone PC10) in blocking buffer overnight at 4°C, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective. -
Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Citrate Buffer 1X pH6.0 at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 2.5 µg/ml of Purified anti-human/mouse/rat PCNA (clone PC10) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® 594 goat anti-mouse secondary antibody (red) for two hours at room temperature. The nuclei were counter staining with DAPI (blue). The image was captured with a 10X objective
Cat # | Size | Price | Quantity Check Availability | Save | ||
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307901 | 25 µg | 48€ | ||||
307902 | 100 µg | 92€ |
The PC10 monoclonal antibody reacts with proliferating cell nuclear antigen also known as PCNA or the DNA polymerase δ auxiliary protein. PCNA is a 36 kD trimeric ring that acts as a DNA-polymerase sliding clamp expressed in the nucleus of all proliferating cells. A prime function of PCNA appears to be increasing DNA polymerase δ processibility during elongation of the leading strand. PCNA is a useful marker for DNA synthesis and is highly conserved among most species, thus highlighting the very broad reactivity of this antibody.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant rat PCNA
- Formulation
- Phosphate-buffered solution, pH7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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ICFC - Quality tested
ICC, IHC-P - Verified
IP, WB, IHC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. Please follow the Cell Fixation and Permeabilization Protocol Using 70% Ethanol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Additional reported applications (for the relevant formats) include: immunohistochemical staining2,5,6 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded tissue sections, immunoprecipitation, intracellular flow cytometry3, immunofluorescence microscopy9, and Western blotting10.
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Application References
(PubMed link indicates BioLegend citation) -
- Ogata K, et al. 1985. J. Immunol. 135:2623.
- Garcia R, et al. 1989. Am. J. Pathol. 134:733.
- Landberg G, et al. 1990. Exp. Cell. Res. 187:111.
- Waseem N, et al. 1990. J. Cell Sci. 96:121.
- Yu C, et al. 1991. Histopathology 19:29.
- Wilkins B, et al. 1992. J. Pathol. 166:45.
- Yang W, et al. 1996. Human Pathol. 27:70.
- Galkowska H, et al. 1996. Vet. Immunol. Immunopathol. 53:329.
- Chou HYE, et al. 2006. J. Biol. Chem. 10:1074.
- Fulvio MD, et al. 2006. Oncogene 25:3932.
- Eswarakumar VP and Schlessinger J. 2007. Proc. Natl. Acad. Sci. USA 104:3937.
- Henkels KM, et al. 2009. Biochem Biophys Res Commun. 389:224. PubMed
- Brobeli A, et al. 2010. Blood Cells Mol Dis. 45:159. PubMed
- Wallace HA, et al. 2014. Development. 141:1332. PubMed
- Mizokami A, et al. 2014. Bone. 69:68. PubMed
- Product Citations
-
- RRID
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AB_314691 (BioLegend Cat. No. 307901)
AB_314692 (BioLegend Cat. No. 307902)
Antigen Details
- Structure
- DNA-polymerase sliding clamp, trimeric ring; 36 kD
- Distribution
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Nuclear, all proliferating cells
- Function
- RAD6-dependent DNA repair pathway; increases DNA polymerase δ processibility during elongation of the leading strand
- Interaction
- PCNA, DNA polymerase δ, Rad6, Rad18, UBC9, MMS2, UBC13, RAD5
- Ligand/Receptor
- Ubiquitination, Sumoylation
- Cell Type
- Neural Stem Cells
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Nuclear Markers
- Antigen References
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1. Travali S, et al. 1989. J. Biol. Chem. 264:7466.
2. Waseem N, et al. 1990. J. Cell Sci. 96:121.
3. Hall P, et al. 1990. J. Pathol. 162:285.
4. Landberg G, et al. 1991. Cancer Res. 51:4570.
5. Woods A, et al. 1991. Histopathol. 19:21.
6. Hoege C, et al. 2002. Nature 419:135.
7. Yue H, et al. 2003. World J. Gastroenterol. 9:377.
8. Shan B, et al. 2003. J. Biol. Chem. 278:44009. - Gene ID
- 5111 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- PCNA
- Specificity Alt (DOES NOT SHOW ON TDS):
- PCNA
- App Abbreviation (DOES NOT SHOW ON TDS):
- ICFC,ICC,IHC,IP,WB
- UniProt
- View information about PCNA on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All PCNA Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-human/mouse/rat PCNA | PC10 | ICFC |
PE anti-human/mouse/rat PCNA | PC10 | ICFC |
Purified anti-human/mouse/rat PCNA | PC10 | ICFC,ICC,IHC,IP,WB |
Alexa Fluor® 488 anti-human/mouse/rat PCNA | PC10 | ICFC |
Alexa Fluor® 647 anti-human/mouse/rat PCNA | PC10 | ICFC |
TotalSeq™-Bn0037 anti-human/mouse/rat PCNA | PC10 | SB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-human/mouse/rat PCNA
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PE anti-human/mouse/rat PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 PE -
Purified anti-human/mouse/rat PCNA
Hela cell nuclear extract was resolved by electrophoresis, t... HeLa cells were fixed with 1% paraformaldehyde (PFA) for 10 ... Human paraffin-embedded intestine tissue slices were prepare... -
Alexa Fluor® 488 anti-human/mouse/rat PCNA
MOLT-4 cells fixed in 70% ethanol then stained with PC10 Ale... -
Alexa Fluor® 647 anti-human/mouse/rat PCNA
Human peripheral blood lymphocytes fixed with 70% ethanol, t... -
TotalSeq™-Bn0037 anti-human/mouse/rat PCNA
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