- Clone
- C9B7W (See other available formats)
- Regulatory Status
- RUO
- Other Names
- LAG3, LAG-3
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Anti-mouse LAG-3, or CD223, inhibits the binding of mouse LAG-3 to Galectin-3. Mouse LAG-3 binds immobilized mouse Galectin-3 in a dose dependent manner (purple circles). Mouse LAG-3 (1 µg/mL) was pre-incubated with increasing concentrations of anti-mouse LAG-3 (clone C9B7W, purple squares) prior to adding to wells containing immobilized mouse Galectin-3. Clone C9B7W inhibits the interaction between LAG-3 and Galectin-3 in a dose dependent manner.
CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine CD223-Ig fusion protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.
- Preparation
- The GoInVivo™ antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.
- Application
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FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment. - Application Notes
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GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.
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Application References
(PubMed link indicates BioLegend citation) -
- Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
- Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
- Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
- RRID
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AB_2566284 (BioLegend Cat. No. 125216)
AB_2566285 (BioLegend Cat. No. 125217)
AB_2566286 (BioLegend Cat. No. 125218)
AB_2566095 (BioLegend Cat. No. 125213)
AB_2566096 (BioLegend Cat. No. 125214)
AB_2566097 (BioLegend Cat. No. 125215)
Antigen Details
- Structure
- Ig superfamily
- Distribution
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T cells and NK cells, as well as T regulatory cells
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Baixeras E et al. 1992. J. Exp. Med. 176:327.
- Gene ID
- 16768 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD223
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD223
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC,FA,IP,ELISA
- UniProt
- View information about CD223 on UniProt.org
Related FAQs
Other Formats
View All CD223 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained ... -
Biotin anti-mouse CD223 (LAG-3)
ConA-stimulated (3days) C57BL/6 splenocytes were stained wit... -
PE anti-mouse CD223 (LAG-3)
ConA-stimulated C57BL/6 splenocytes stained with C9B7W PE -
APC anti-mouse CD223 (LAG-3)
Con A-stimulated (day-3) C57BL/6 splenocytes stained with C9... -
PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3)
Con A-stimulated splenocytes stained with anti-mouse CD223 P... -
Brilliant Violet 785™ anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
GoInVivo™ Purified anti-mouse CD223 (LAG-3)
Anti-mouse LAG-3, or CD223, inhibits the binding of mouse LA... -
Brilliant Violet 421™ anti-mouse CD223 (LAG-3)
Con A + IL-2-stimulated C57BL/6 splenocytes (3 days) were st... -
PE/Dazzle™ 594 anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
Brilliant Violet 650™ anti-mouse CD223 (LAG-3)
Con A + IL-2-stimulated C57BL/6 splenocytes (3 days) were st... -
PE/Cyanine7 anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stai... -
TotalSeq™-A0378 anti-mouse CD223 (LAG-3)
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Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained ... -
TotalSeq™-C0378 anti-mouse CD223 (LAG-3)
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APC/Fire™ 750 anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
Alexa Fluor® 647 anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
TotalSeq™-B0378 anti-mouse CD223 (LAG-3)
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Brilliant Violet 711™ anti-mouse CD223 (LAG-3)
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes were sta... -
PE/Fire™ 640 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
PerCP/Fire™ 806 anti-mouse CD223 (LAG-3)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
PerCP/Fire™ 780 anti-mouse CD223 (LAG-3)
Con-A and IL-2 stimulated C57BL/6 splenocytes (three days) w... -
Spark Red™ 718 anti-mouse CD223 (LAG-3) (Flexi-Fluor™)
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PE/Cyanine5 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
PE/Fire™ 810 anti-mouse CD223 (LAG-3)
ConA+ IL-2 stimulated (3 days) C57BL/6 splenocytes were stai... -
Brilliant Violet 605™ anti-mouse CD223 (LAG-3)
Con A+IL-2-stimulated (3 days) C57BL/6 splenocytes were stai...
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