- Clone
- R73 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TCR α/β, α/β TCR
- Isotype
- Mouse (BALB/c) IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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LOU rat splenocytes stained with R73 Alexa Fluor® 647
Cat # | Size | Price | Quantity Check Availability | Save | ||
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201116 | 100 µg | 184€ |
TCR α and β are members of the immunoglobulin superfamily and components of the CD3/TCR complex expressed on most peripheral T cells, intestinal epithelial lymphocytes, and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation. The TCR α/β recognizes peptide bound to MHC class I and II.
Product DetailsProduct Details
- Verified Reactivity
- Rat
- Reported Reactivity
- Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Rat T cell blasts & erythrocytes
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, immunohistochemical staining of acetone-fixed frozen sections1, in vitro activation of α/β TCR+ T cells 1,2 and in vivo depletion2 of α/β TCR+ T cells . The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 201112).
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Application References
(PubMed link indicates BioLegend citation) -
- Hunig T, et al. 1989. J. Exp. Med. 169:73.
- Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
- Brenan M, et al. 1997. Eur. J. Immunol. 27:3070.
- Yoshino N, et al. 2000. Exp. Anim. 49:97.
- Product Citations
-
- RRID
-
AB_572055 (BioLegend Cat. No. 201116)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR complex with CD3 and TCR-α subunit, 40 and 46 kD
- Distribution
-
Most peripheral T cells, intestinal epithelial lymphocytes, thymocytes
- Function
- Antigen recognition, T cell activation/responses
- Ligand/Receptor
- Peptide bound-MHC class I and II
- Cell Type
- Lymphocytes, T cells, Thymocytes
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Hunig T, et al. 1989. J. Exp. Med. 169:73.
2. Fangmann J, et al. 1991. Eur. J. Immunol. 21:753.
3. Mitnacht R, et al. 1995. Eur. J. Immunol. 25:328.
4. Peiters RH, et al. 1995. Biochem. Biophys. Res. Commun. 214:552.
5. Brenan M, et al. 1997. Eur. J. Immunol. 27:3070. - Gene ID
- 24820 View all products for this Gene ID 290071 View all products for this Gene ID
- UniProt
- View information about TCR alphabeta on UniProt.org
Other Formats
View All TCR α/β Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-rat TCR α/β | R73 | FC |
FITC anti-rat TCR α/β | R73 | FC |
PE anti-rat TCR α/β | R73 | FC |
Alexa Fluor® 647 anti-rat TCR α/β | R73 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-rat TCR α/β
LOU rat splenocytes stained with R73 APC -
FITC anti-rat TCR α/β
LOU rat splenocytes stained with R73 FITC -
PE anti-rat TCR α/β
LOU rat splenocytes stained with R73 PE -
Alexa Fluor® 647 anti-rat TCR α/β
LOU rat splenocytes stained with R73 Alexa Fluor® 647
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