Biotin anti-human EPO Antibody

Pricing & Availability
Clone
M038G10 (See other available formats)
Other Names
Hematopoietin
Isotype
Mouse IgG2b, κ
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Cat # Size Price Quantity Avail. Save
502704 500 µg 320€
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Description

Erythropoietin (EPO) is the most important hormone regulating erythropoiesis. It is a glycoprotein also known as hematopoietin. Human EPO is mainly produced in the kidney and liver in response to hypoxia to induce red blood cell synthesis. EPO is a secreted protein of 166 amino acids with a predicted molecular mass of approximately 18 kD. However, it migrates on SDS-PAGE at about 35-55 kD due to heavy glycosylation. Human EPO is closely related to thrombopoietin (TPO), sharing 44% amino acid homology. EPO is highly conserved across species, with human EPO being 80% identical to mouse, and mouse EPO being 95% identical to rat.

 

EPO functions by binding to its receptor, EPO R, a homodimer, on erythoid precursors, and promotes their differentiation and maturation. As expected, serum levels of EPO are elevated in response to various conditions that result in tissue hypoxia (e.g. blood loss, exposure to high altitude, cardiac or pulmonary disease, and anemia). Increased EPO levels can also be due to increased production by cancer cells of the liver and kidney. Decreased levels of EPO are often seen with chronic renal failure and other chronic diseases or chemotherapy, which cause anemia. Human endogenous EPO and recombinant EPO share the same amino acid sequence, with differences in the glycosylation profiles.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
In-house recombinant mouse EPO.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA. For ELISA detection applications, a concentration of 0.5 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of EPO recombinant protein ranging from 500 to 7.81 mIU/mL is recommended for each ELISA plate.

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated M038G10 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified M0312E3 (Cat. No. 662202) antibody as the capture antibody.

 

Note: For testing human EPO in cell culture supernatant, serum, plasma (heparin, EDTA and citrate), urine, and other biological fluids, BioLegend's LEGEND MAX™ Kits (Cat. No. 442907 & 442908) are specially developed and recommended.

RRID
AB_2650709 (BioLegend Cat. No. 502704)

Antigen Details

Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
2056 View all products for this Gene ID
UniProt
View information about EPO on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 04.03.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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