Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Raised against granulocytes of mouse origin

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested
IP, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C¹⁹. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A8¹⁹. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.4²⁰.

The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity², in vivo depletion^3-5,9, immunoprecipitation¹, immunohistochemical staining⁶ (including paraffin-embedded sections^9,16,33-35, acetone-fixed frozen sections¹¹ and zinc-fixed sections¹⁵), and Western blotting⁷. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)²⁰.

Special Note: For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436).

Application References

(PubMed link indicates BioLegend citation)

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
2. Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
3. Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
4. Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
5. Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
6. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
7. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
8. Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
9. Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
10. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
11. Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
12. Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
13. Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
14. Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
15. Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
16. Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
17. Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
18. Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
19. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
20. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
21. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
22. Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
23. Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
24. Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
25. Collins C, et al. 2014. PNAS. 111:9899. PubMed
26. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
27. Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
28. Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
29. Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
30. Distel E, et al. 2014. Circ Res. 115:759. PubMed
31. Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
32. Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
33. Whiteland J, et al. 1994 J Histochem Cytochem 43:3 (IHC-P)
34. Brown C, et al. 2003 J Immunology 171:2 (IHC-P)
35. Obregon-Henao A, et al. PLoS One 8:11 (IHC-P)

Product Citations

1. Ahmed AU, et al. 2017. J Immunol. 199(6):2128. PubMed
2. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
3. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
4. Zhu C, et al. 2018. Sci Rep. 5.113194444. PubMed
5. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
6. Tanimura N et al. 2018. Developmental cell. 46(5):581-594 . PubMed
7. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
8. Bouchareychas L, et al. 2021. iScience. 24(8):102847. PubMed
9. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
10. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
11. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
12. Liu M, et al. 2022. J Exp Med. 219:. PubMed
13. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
14. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
15. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
16. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
17. Dubeykovskaya Z, et al. 2016. Nat Commun. 7:10517. PubMed
18. Preglej T, et al. 2020. JCI Insight. 5(4):. PubMed
19. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
20. Gutiérrez-Gutiérrez Ó, et al. 2021. EMBO Rep. 22:e52905. PubMed
21. Scalzo S, et al. 2022. Int J Nanomedicine. 17:2865. PubMed
22. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
24. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
25. Hinterbrandner M, et al. 2021. JCI Insight. 6:e151797. PubMed
26. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
27. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
28. Davis FM, et al. 2019. Arterioscler Thromb Vasc Biol. 39:2353. PubMed
29. Mikami H, et al. 2020. Nat Commun. 11:1162. PubMed
30. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
31. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
32. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
33. Wu HC, et al. 2021. Cancer Discov. Online ahead of print. PubMed
34. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
35. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
36. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
37. Olsson A, et al. 2016. Nature. 10.1038/nature19348. PubMed
38. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
39. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
40. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
41. Parada-Kusz M, et al. 2018. Dis Model Mech. 11:. PubMed
42. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
43. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
44. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
45. Getts D, et al. 2008. J Exp Med. 205:2319. PubMed
46. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
47. Yu VWC, et al. 2017. Cell. 168:944. PubMed
48. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
49. Luo W et al. 2018. Immunity. 48(2):313-326 . PubMed
50. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
51. Köchl R, et al. 2020. Elife. 9:00. PubMed
52. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
53. Kotaki R, et al. 2020. Sci Rep. 10:13554. PubMed
54. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
55. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
56. Progatzky F, et al. 2021. Nature. 599:125. PubMed
57. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
58. Ishidome T et al. 2017. EBioMedicine. 22:89-99 . PubMed
59. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
60. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
61. Perez E, et al. 2020. J Immunol. 205:2489. PubMed
62. Aktories P, et al. 2022. Cell Rep Methods. 2:100260. PubMed
63. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
64. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
65. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
66. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
67. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
68. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
69. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
70. Goldstein JM et al. 2019. Cell reports. 27(4):1254-1264 . PubMed
71. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
72. Gao Y, et al. 2020. Immunity. 52(6):1007-1021.e8. PubMed
73. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
74. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
75. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
76. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
77. Zeis P, et al. 2020. Immunity. 53:775. PubMed
78. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
79. Nagashima H et al. 2019. Immunity. 51(4):682-695 . PubMed
80. Shin JW, et al. 2020. Cell Death Dis. 0.667361111. PubMed
81. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
82. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
83. Yokomizo-Nakano T, et al. 2020. Cancer Res. 80:2523. PubMed
84. Roderick J, et al. 2014. Proc Natl Acad Sci U S A. 111:14436. PubMed
85. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
86. Zhang C, et al. 2018. Cell Res. 28:323. PubMed
87. Michel A, et al. 2013. J Immunol. 90:5534. PubMed
88. Lefkopoulos S, et al. 2020. Immunity. 53(5):934-951.e9. PubMed
89. Mohrin M, et al. 2021. Aging Cell. 20:e13313. PubMed
90. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
91. Wagner AK, et al. 2022. iScience. 25:105137. PubMed
92. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
93. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
94. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
95. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
96. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
97. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
98. Morganti C, et al. 2022. EMBO Rep. 23:e54262. PubMed
99. Heyde A, et al. 2021. Cell. 184(5):1348-1361.e22. PubMed
100. Li ZG, et al. 2020. Chin Med J (Engl). 1943:133. PubMed
101. Teng Y, et al. 2021. Mol Ther. 29:2424. PubMed
102. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
103. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
104. Olariu V, et al. 2021. Cell Reports. 34(2):108622. PubMed
105. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed

RRID

AB_313368 (BioLegend Cat. No. 108403)
AB_313369 (BioLegend Cat. No. 108404)

Antigen Details

Structure

21-25 kD

Distribution

Granulocytes, monocytes

Cell Type

Granulocytes, Monocytes, Neutrophils

Biology Area

Immunology, Innate Immunity

Antigen References

1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.

Gene ID

17067 View all products for this Gene ID 546644 View all products for this Gene ID

UniProt

View information about Ly-6G Ly-6C on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

How many biotin molecules are per antibody structure?

We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

Certificate of Analysis