ELISA MAX™ Deluxe Set Human Granzyme B

Pricing & Availability
Regulatory Status
RUO
Other Names
Granzyme B, Granzyme-2, GraynzymeB, GRB, GrzB, GZMB
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Product Citations
publications
Elisa-Max_Human-Granzyme-B-ELISA-Kit_073124
  • Elisa-Max_Human-Granzyme-B-ELISA-Kit_073124
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439204 5 plates 336€
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Description

Granzyme B is a member of the granzyme family of serine proteases found specifically in granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Perforin/granzyme pathway is a molecular weapon for the defense against virus-infected and malignantly transformed host cells. Perforin protein creates a pore in the membrane of targets cells and facilitate the entry of granzymes. This results in results in the translocation of Granzyme B into the nucleus and initiation of apoptosis. Other granzymes have also been implicated in apoptosis, but Granzyme B appears to be the most potent. Human Granzyme B is a potential immunoconjugate and an important facilitator in chronic inflammation and wound healing.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human Granzyme B ELISA MAX™ Capture Antibody (200X) 
  • Human Granzyme B ELISA MAX™ Detection Antibody (200X) 
  • Human Granzyme B Standard 
  • Avidin-HRP (1000X) 
  • Substrate Solution F 
  • Coating Buffer B (5X) 
  • Blocking Buffer A (2X) 
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
0.5 pg/mL
Standard Range
200 - 3.1 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended 
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20 
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4 
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended 
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4 , 0.2 g KH2PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered 
  • Deionized (DI) water 
  • A microplate reader capable of measuring absorbance at 450 nm 
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL 
  • Wash bottle or automated microplate washer 
  • Log-Log graph paper or software for data analysis 
  • Tubes to prepare standard dilutions 
  • Timer

Antigen Details

Cell Type
B cells, Dendritic cells, NK cells, T cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Cytokines/Chemokines, Enzymes and Regulators, Proteases, Soluble Receptors
Gene ID
NA
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 07.31.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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