ELISA MAX™ Deluxe Set Human Soluble CTLA-4

Pricing & Availability
Regulatory Status
RUO
Other Names
CD152, cluster of differentiation 152
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Product Citations
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ELISA-MAX-Deluxe-Set_Human-Soluble-CTLA-4_060724
ELISA MAX™ Deluxe Set Human Soluble CTLA-4 Standard Curve
  • ELISA-MAX-Deluxe-Set_Human-Soluble-CTLA-4_060724
    ELISA MAX™ Deluxe Set Human Soluble CTLA-4 Standard Curve
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437404 5 plates 336€
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Description

CTLA4 (Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein that plays an important regulatory role in the immune system. CTLA4 is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. Mutations in the CTLA4 gene have been associated with various autoimmune diseases, such as systemic lupus erythematosus, insulin-dependent diabetes mellitus, and other autoimmune diseases. sCTLA-4 immunoreactivity was inhibited by its binding to B7.1, suggesting that sCTLA-4 is a functional receptor. sCTLA4 also plays a role in the initial immune response to infection of immune cells by HIV, along with the CD-1 pathway and others.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Human sCTLA-4 Capture Ab (200X)
  • Human sCTLA-4 Detection Ab (200X)
  • Human sCTLA-4 Standard
  • Avidin-HRP (1000X)
  • Substrate Solution F
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)
  • Blocking Buffer A (2X)

Product Details

Verified Reactivity
Human
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Sensitivity
11.7 ± 3.6 pg/mL
Standard Range
54.7 - 3500 pg/mL
Materials Not Included
  • Microwell plates: BioLegend Cat. No. 423501 is recommended
  • Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
  • Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
  • Plate Sealers: BioLegend Cat. No. 423601 is recommended
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
  • Deionized (DI) water
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer

Antigen Details

Cell Type
Lymphocytes, Schwann Cells, Tregs
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology, Immunology
Molecular Family
Immune Checkpoint Receptors
Gene ID
1493 View all products for this Gene ID
UniProt
View information about CTLA-4 on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 1    Revision Date: 06.07.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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