LEGEND MAX™ Human IL-23 ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Interleukin-23, IL23 Precoated ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
LEGENDMAX_Human_IL-23_ELISA_062917
  • LEGENDMAX_Human_IL-23_ELISA_062917
Cat # Size Price Quantity Check Availability Save
437607 1 Pre-coated Plate 370€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

IL-23 is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23 and a p40 subunit that is shared with IL-12. IL-23 is secreted by activated macrophages and dendritic cells. Human and mouse p19 share 70% amino acid sequence identity. IL-23 has been shown to enhance IFN-? production by memory T cells. IL-23 is upregulated in certain autoimmune diseases and promotes immunity in response to some viral and mycobacterial infections. The cytokine has also been implicated in inflammatory diseases such as rheumatoid arthritis and Crohn’s disease.

BioLegend’s Human IL-23 LEGEND MAX™ ELISA Kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with 96 well microtiter strip plates that are pre-coated with a capture antibody.

This kit is specifically designed for the accurate quantitation of IL-23 from cell culture supernatant, serum, plasma, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human IL-23 Pre-coated 96-well Strip Microplate
  • Human IL-23 Dectection Antibody
  • Human IL-23 Standard
  • Avidin-HRP
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA

Product Citations
  1. Izati AF, et al. 2021. Front Immunol. 12:690908. PubMed
  2. Ji J, et al. 2021. Front Immunol. 12:787565. PubMed
Sensitivity
2.45 pg/mL
Standard Range
12.5-800 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Gene ID
51561 View all products for this Gene ID
UniProt
View information about IL-23 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 1    Revision Date: 06.29.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account