- Clone
- H57-597 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TCR-β chain, TCR-β, β-TCR
- Isotype
- Armenian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![H57-597_Blue_042909.jpg H57-597_Blue_042909.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/H57-597_Blue_042909.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 splenocytes stained with H57-597 Pacific Blue™
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
109225 | 25 µg | 76€ | ||||
109226 | 100 µg | 172€ |
T cell receptor (TCR) is a heterodimer consisting of an α and a β chain (TCR α/β) or a γ and a δ chain (TCR γ/δ). TCR-β is a member of the immunoglobulin superfamily and a component of the CD3/TCR complex (along with TCR-α). It is expressed on α/β TCR-bearing T cells and thymocytes. The CD3/TCR complex plays a key role in antigen recognition, signal transduction, and T cell activation.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Affinity purified TCR from mouse DO-11.10 cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
H57-597 is a hamster mAb directed to an epitope of the C region of TCR ß chain12. The H57-597 antibody does not cross-react with ?/d TCR-bearing T cells. Immobilized or soluble H57-597 antibody can activate a/ß TCR-bearing T cells. Additional reported applications (for the relevant formats) for this antibody include: immunoprecipitation2, in vitro stimulation2,3, in vivo depletion4-6, and immunohistochemical staining of acetone-fixed frozen sections7,8,9. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 109253-109258).
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Application References
(PubMed link indicates BioLegend citation) -
- Gascoigne NJ. 1990. J. Biol. Chem. 265:9296.
- Kruisbeek A, et al. 1991. In Current Protocols in Immunology. pp. 3.12.1. (Costim IP)
- Davenport C, et al. 1995. J. Immunol. 155:3742. (Costim)
- Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
- Kummer U, et al. 2001. Immunol. Lett. 75:153. (Deplete)
- van der Heyde HC, et al. 1995. J. Immunol. 154:3985. (Deplete)
- Tomita K, et al. 1999. Genes Dev. 13:1203. (IHC)
- Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC)
- Ponomarev ED, et al. 2007. J. Immunol. 178:39. (IHC)
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
- Tsukumo S, et al. 2006. J.Immunol. 177:8365. (FC) PubMed
- Grégoire C, et al. 1991. Proc. Natl. Acad. Sci USA 88:8077.
- Product Citations
-
- RRID
-
AB_1027655 (BioLegend Cat. No. 109225)
AB_1027649 (BioLegend Cat. No. 109226)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR complex with CD3 and TCR α subunit
- Distribution
-
Majority of T cells and thymocytes (correlated to differentiation)
- Function
- Antigen recognition, T cell activation
- Ligand/Receptor
- Peptide bound-MHC class I and II
- Antigen References
-
1. Davis MM, et al. 1998. Ann. Rev. Immunol. 16:523.
2. Huppa JB, et al. 2003. Nat. Immunol. 4:749.
3. Kubo R, et al. 1989. J. Immunol. 142:2736. - Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR beta chain on UniProt.org
Related Pages & Pathways
Pages
Other Formats
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 APC -
Biotin anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with biotinylated H57-597,... C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
FITC anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 FITC -
PE anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained with H57-597 PE and TCRγ/δ ... -
PE/Cyanine5 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine5. -
Purified anti-mouse TCR β chain
C57L/6 splenocytes stained with H57-597 purified, followed b... Fresh, frozen mouse spleen was stained with purified TCR β c... -
Alexa Fluor® 488 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor® ... -
Alexa Fluor® 647 anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Alexa Fluor® 647 C57BL/6 mouse frozen spleen section was fixed with 4% parafo... -
APC/Cyanine7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
PE/Cyanine7 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 PE/Cyanine7 -
Alexa Fluor® 700 anti-mouse TCR β chain
C57BL/6 mouse splenocytes stained with H57-597 Alexa Fluor&r... -
Pacific Blue™ anti-mouse TCR β chain
C57BL/6 splenocytes stained with H57-597 Pacific Blue™ -
Brilliant Violet 421™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
PerCP/Cyanine5.5 anti-mouse TCR β chain
C57BL/6 splenocytes were stained with CD3e APC and TCR ß cha... -
Brilliant Violet 570™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
Brilliant Violet 510™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
Purified anti-mouse TCR β chain (Maxpar® Ready)
Mouse splenocytes stained with 143Nd-anti-TCRβ (H57-597) and... -
Alexa Fluor® 594 anti-mouse TCR β chain
C57BL/6 mouse frozen lymph node sections were fixed with 4% ... C57BL/6 mouse splenocytes were stained with TCR β (clone H57... -
PE/Dazzle™ 594 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR-β (clon... -
Brilliant Violet 605™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
Brilliant Violet 711™ anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with TCR β (clon... -
APC/Fire™ 750 anti-mouse TCR β chain
C57BL/6 splenocytes were stained with TCR γ/δ PE... -
TotalSeq™-A0120 anti-mouse TCR β chain
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Brilliant Violet 785™ anti-mouse TCR β chain
C57BL/6 splenocytes were stained with CD3 APC and anti-mouse... -
Brilliant Violet 650™ anti-mouse TCR β chain
C57BL/6 Splenocytes were stained with CD3 APC and anti-mouse... -
Ultra-LEAF™ Purified anti-mouse TCR β chain
C57BL/6 CD3+ splenocytes stained with H57-597 PE ... -
TotalSeq™-C0120 anti-mouse TCR β chain
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TotalSeq™-B0120 anti-mouse TCR β chain
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Spark Red™ 718 anti-mouse TCR β chain (Flexi-Fluor™)
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Spark PLUS UV™ 395 anti-mouse TCR β chain
C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε ...
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