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PerCP/Cyanine5.5 anti-Pan-Cytokeratin

Pricing & Availability
Clone
AE-1/AE-3 (See other available formats)
Regulatory Status
RUO
Other Names
Pan-Cytokeratin (AE-1/AE-3), AE1/AE3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. AE-1-AE-3_Percp-Cya55_Pan_ICFC-1_072924
MCF-7 cells (positive cell line, solid histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PerCP/Cyanine5.5 anti-Pan-Cytokeratin (clone AE-1/AE-3) or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (negative control, dashed histogram) (Cat No. 400149).
  • A. AE-1-AE-3_Percp-Cya55_Pan_ICFC-1_072924
    MCF-7 cells (positive cell line, solid histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PerCP/Cyanine5.5 anti-Pan-Cytokeratin (clone AE-1/AE-3) or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (negative control, dashed histogram) (Cat No. 400149).
  • B. AE-1-AE-3_Percp-Cya55_Pan_ICFC_072924
    Jurkat cells (negative cell line, solid histogram) were fixed and permeabilized using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) and intracellularly stained with PerCP/Cyanine5.5 anti-Pan-Cytokeratin (clone AE-1/AE-3) or PerCP/Cyanine5.5 mouse IgG1, κ isotype control (dashed histogram) (Cat No. 400149).
Compare all formats See PerCP/Cyanine5.5 spectral data
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914209 25 tests 144€
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914210 100 tests 360€
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Description

AE-1 immunoreacts with an antigenic determinant present on most of the subfamily A cytokeratins, including cytokeratins with molecular weights of 56.5, 50, 48 and 40 kD. AE-3 reacts with an antigenic determinant shared by the subfamily B cytokeratins including cytokeratins with molecular weights of of 64, 59, 58, 56, and 52 kD.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Reported Reactivity
Dog, Non-Human Primate
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was developed using human epidermal keratins.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm. 

Excitation Laser
Blue Laser (488 nm)
Additional Product Notes

For use of this antibody in intracellular flow cytometry (ICFC), fixation/permeabilization using Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803) is recommended.

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
NA
UniProt
View information about Pan-Cytokeratin on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 1    Revision Date: 07.29.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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