Purified anti-CD34 Antibody (Previously Covance catalog# SIG-3326)

Pricing & Availability
Clone
QBEnd/10 (See other available formats)
Regulatory Status
RUO
Other Names
Hematopoietic progenitor cell antigen CD34, CD34 antigen
Previously
Signet Catalog# 326-26 L2 Predilute
Covance Catalog# SIG-3326
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_QBEndslash10_Purified_CD34_Antibody_IHC_021715
Robust cytoplasmic and membrane staining of endothelial cells in frozen human placenta with antibody CD34.
  • A_QBEndslash10_Purified_CD34_Antibody_IHC_021715
    Robust cytoplasmic and membrane staining of endothelial cells in frozen human placenta with antibody CD34.
  • B_QBEndslash10_PURE_CD34_Antibody_111423
    SeqIF™ (sequential immunofluorescence) staining on COMET™ of Purified anti-CD34 (clone QBEnd/10, yellow) on formalin-fixed paraffin-embedded human pancreatic carcinoma tissue at 1.25 µg/mL. Alexa Fluor™ Plus 647 Goat anti-Mouse IgG antibody (Lunaphore, Cat. No. DR647MS) was used as a secondary antibody. Nuclei were counterstained with DAPI (blue). Tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing.
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826401 50 µL 168€
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Description

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated using a vesicular suspension of a perfusate of human term placenta.
Formulation
Phosphate-buffered solution with BSA + 0.09% NaN3.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

IHC - Quality tested
SB - Community verified

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining.

The optimal working dilution should be determined for each specific assay condition.
IHC: 1:1000-1:1500 (concentrated format) with Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).

Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 20 minutes at room temperature

Application Notes

This antibody is effective in immunohistochemistry (IHC).

The BioLegend QBEnd/10 monoclonal antibody detects the CD34 antigen. It stains the cytoplasm of endothelial cells of vascular tissue with some cross reactivity to basement membrane collagen. In tumors this monoclonal antibody is valuable in labelling the vascular endothelium of Kaposi's sarcoma, tumors involving the liver, spindle shaped tumors and any other highly vascular neoplastic or normal tissue.

**We recommend a quick spin prior to opening the vial.
Positive tissue (human): Skin, tonsil or other highly vascular tissue.

Additional Product Notes

For the use of this antibody in spatial biology applications, we have partnered with Lunaphore Technologies for demonstration of our antibodies on the COMET™. The COMET™ platform is an automated, end-to-end spatial biology solution developed for rapid and flexible multiplex tissue profiling. More information on the COMET™ and a complete list of our antibodies that have been demonstrated on the COMET™ can be found here.

Application References

(PubMed link indicates BioLegend citation)
  1. Anthony P, et al. Endothelial markers in malignant vascular tumor of the liver: superiority of QBEnd/10 over von willebrand factor and uelex europus agglutinin 1. J Clin Path 44:2, 1991.
  2. Sankey EA, et al. QBEnd/10: a new immunostain for the diagnosis of Kaposi's Sarcoma. J Path 161:267-271, 1990.
  3. Fletcher CDM, et al. QBEnd/10: a useful but by no means a specific marker for the diagnosis of kaposi's sarcoma. J Path 162:274, 1990.
  4. Ramani P, et al. QBEnd/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathol 17:237-242, 1990.
Product Citations
  1. Jeon SB, et al. 2023. Cell Prolif. 56:e13366. PubMed
  2. Jeon SB, et al. 2022. Int J Stem Cells. Online ahead of print. PubMed
  3. Verginadis II, et al. 2022. Nat Cell Biol. 24:940. PubMed
RRID
AB_2564903 (BioLegend Cat. No. 826401)

Antigen Details

Structure
105-120 kD single chain mucin-like glycoprotein
Distribution

Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts

Function
Cell adhesion
Ligand/Receptor
L-selectin, E-selectin
Cell Type
Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Antigen References

1. Krause DS, et al. 1996. Blood 87:1.
2. Puri KD, et al. 1995. J. Cell Biol. 131:261.
3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.

Gene ID
947 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?

It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.

Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?

Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.

Are other fluorophores compatible with IBEX?

Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.

The same antibody works in one tissue type but not another. What is happening?

Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.

How can I be sure the staining I’m seeing in my tissue is real?

In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.

Go To Top Version: 4    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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