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Purified anti-TP63 Antibody

Pricing & Availability
Clone
W15093A (See other available formats)
Regulatory Status
RUO
Other Names
Tumor protein p63, p63, Chronic ulcerative stomatitis protein (CUSP), Keratinocyte transcription factor (KET), Tumor protein p73-like (p73L), TP63
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
1_W15093A_PURE_TP63_Antibody_1_WB_111617_Updated
15 µg total protein from NIH3T3 (lane 1), HaCat (lane 2) and 10 µg total protein from 293E overexpressed with TA-p63α (lane 3) and ΔN-p63α (lane 4) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) or 1:106 (0.0005 µg/ml) purified anti-TP63 antibody, clone W15093A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder. This antibody recognizes more than one isoform of ΔN-p63 which might be detected lower than 75kD.
  • 1_W15093A_PURE_TP63_Antibody_1_WB_111617_Updated
    15 µg total protein from NIH3T3 (lane 1), HaCat (lane 2) and 10 µg total protein from 293E overexpressed with TA-p63α (lane 3) and ΔN-p63α (lane 4) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) or 1:106 (0.0005 µg/ml) purified anti-TP63 antibody, clone W15093A (upper). Proteins were visualized using chemiluminescence detection by incubation with HRP goat anti-mouse-IgG secondary antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M is the Molecular weight ladder. This antibody recognizes more than one isoform of ΔN-p63 which might be detected lower than 75kD.
  • 2_W15093A_GoChIP_TP63_Antibody_1_050918.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HaCaT cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 Antibody (clone W15093A, Cat. No. 687203) or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody (Clone MG2b-57, Cat. No. 401201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human CDKN1A gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • 3_W15093A_GoChIP_TP63_Antibody_1_050918.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of HaCaT cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-TP63 Antibody (clone W15093A, Cat. No. 687203) or B) equal amount of Purified Mouse IgG2b, κ Isotype Control Antibody (Clone MG2b-57, Cat. No. 401201) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human MDM2 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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687202 100 µg 278€
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Description

TP63 is a p53-related gene and shares similar domain organization with p53 and p73. Full length TP63 contains a N-terminal transactivation domain, a central DNA binding domain, and a C-terminal oligomerization domain responsible for the formation of a homotetramer. TP63 have several isoforms produced by alternative splicing. Most of the isoforms contain the DNA binding domain, but differ in their transactivation and oligomerization domains. The transactivation domain is present in the transcriptional active isoforms (TA-p63) and is absent in the truncated isoforms (ΔN -p63). TP63 is expressed in keratinocyte stem cells and is essential for the morphogenesis and development of ectodermal, orofacial, and limb tissues. TP63 deficient mice die soon after birth with skin and limb developmental defects.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human TP63 recombinant protein (1-250 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ChIP - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL (1:250-1:1000 dilution). For ChIP applications, the suggested dilution is 1:50 - 1:200 by volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Application Notes: This clone predominantly recognizes ?N-p63, and weakly crosses react with TA-p63. Both  ?N-p63 and TA-p63 have multiple isoforms, the largest isoforms are 66kD and 77kD respectively. This antibody recognizes more than one isoform of ?N-p63.

Product Citations
  1. Senosain MF, et al. 2021. Sci Rep. 11:14424. PubMed
  2. Mohibi S, et al. 2020. J Invest Dermatol. 140:2166. PubMed
  3. Zhang Y et al. 2018. Cell stem cell. 23(4):516-529 . PubMed
RRID
AB_2616941 (BioLegend Cat. No. 687202)

Antigen Details

Structure
p53 family of transcription factors. 12 reported isoforms produced by alternative splicing. Predicted molecular weight ranged from 44.7kD to 76.8kD.
Distribution

Nuclear.

Function
TP63 acts as a transcriptional activator or repressor, is essential for limb formation, epidermal morphogenesis, and epithelial stem cell regeneration.
Interaction
Forms a homotetramer. Interacts with HIPK2, SSRP1, WWP1, PDS5A, and NOC2L.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Katoh I, et al. 2016. Cell Cycle 15:699.
2. Candi E, et al. 2014. Hum. Mutat. 35:702.
3. Chari NS, et al. 2013. Cell Death Differ. 20:1080.
4. Vasmatzis G, et al. 2012. Blood 120:2280.
5. Scott DW, et al. 2012. Blood 119:4949.
6. Miki D, et al. 2010. Nat. Genet. 42:893.

Gene ID
8626 View all products for this Gene ID
UniProt
View information about TP63 on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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