Recombinant Human IL-7 (E. coli expressed, carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
IL7, IL-7, interleukin-7, Interleukin 7, Lymphopoietin-1 (LP-1), PBGF
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Product Citations
publications
a.
Recom_Human-IL-7_E-coli_1_CF
Human IL-7 induces proliferation of PHA-activated human peripheral blood lymphocytes (PBL) in a dose-dependent manner. The ED50 for this effect is 0.1 – 1.5 ng/mL.
  • a.
Recom_Human-IL-7_E-coli_1_CF
    Human IL-7 induces proliferation of PHA-activated human peripheral blood lymphocytes (PBL) in a dose-dependent manner. The ED50 for this effect is 0.1 – 1.5 ng/mL.
  • b.
Recom_Human-IL-7_E-coli_2_CF
    Stability Testing for Recombinant Human IL-7. Recombinant human IL-7 was aliquoted in PBS and pH 7.2 at 0.2 mg/mL. One aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to induce the proliferation of PHA-activated human PBL in a dose-dependent manner. The ED50 for this effect is 0.1 – 1.5 ng/mL.
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569704 25 µg 340€
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569706 100 µg 864€
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Description

IL-7 was initially described as a stromal derived factor capable of inducing in vitro growth of pre-B cells. IL-7 acts on a variety of cells through its receptor (IL-7R), a heterodimer consisting of IL-7Rα (CD127) and a common γc chain (CD132) shared by other cytokine (IL-2, IL-4, IL-9, IL-15, and IL-21) receptors. The generation of IL-7-deficient and IL-7Rα-deficient mice and monoclonal antibody blocking experiments confirmed the requirement of IL-7 for B-cell development in mice. Mutations in the α chain of the IL-7 receptor in patients with severe combined immunodeficiency (SCID) confirmed that IL-7 is indispensable for T-cell development in humans. However, the presence of B cells in these individuals suggests important differences between the role of IL-7 in murine and human lymphocyte development. Thus, although human B-cell development does not appear to require IL-7, immature human B cells do proliferate in response to IL-7. Recent information suggests that IL-7 dependence in human lymphopoiesis increases during the progression of ontogeny in cord blood and bone marrow. Relative to IL-7 alone, greater proliferation of CFUs, SLPs and pre-pro B cells is observed by hybrid cytokine IL-7/HGFβ, as association of IL-7 and hepatocyte growth factor (HGFβ). This hybrid cytokine signals through both IL-7R (IL-7Rα plus γc) and c-Met. IL-7 has a thymic antiapoptotic effect and induces the expression of antiapoptotic proteins Bcl-2 and Mcl-1 and the inhibition of proapoptotic proteins Bax and Bad. In addition, IL-7 is a key regulator of glucose uptake in T lymphocytes. TGF-β has been shown to down-regulate IL-7 mRNA and protein secretion from human bone marrow stromal cells. In addition, TGF-β inhibits IL-7-induced proliferation of pre-B cells.

Product Details
Technical data sheet

Product Details

Source
Recombinant human IL-7, amino acid Asp26-His177 (Accession No. P13232) was expressed in E. Coli. The N-terminal includes 6His.
Molecular Mass
The 171 amino acid recombinant protein has a predicted molecular mass of approximately 19.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 15 - 27 kD by SDS-PAGE. The N-terminal contains a 6His tag.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS and pH 7.2
Endotoxin Level
Less than 0.1 EU per µg protein as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
ED50 = 0.1 – 1.5 ng/mL, as determined by a dose-dependent stimulation in a proliferation assay with PHA-activated human peripheral blood lymphocytes (PBL). Deep Blue Cell Viability™ Kit (Cat. No. 424701) is used to measure the proliferation. Results may vary depending on human donors.
Application

Bioassay

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Monomer
Distribution

IL-7 is produced by epithelial cells in thymus, bone marrow, and intestine. Additional sites of IL-7 production include epithelial goblet cells, keratinocytes, fetal liver, adult liver, dendritic cells, skeletal muscle cells, fibroblastic reticular cells, and follicular dendritic cells

Function
IL-7 induces proliferation of human immature B cells, critical for T-cell development and maintenance of peripheral T-cell homeostasis
Interaction
IL-7Rα is expressed on immature B cells through the early pre-B stage. The IL-7Rα is widely expressed in T cells, CD4, CD8 naÏve and memory cells, and dendritic cells
Ligand/Receptor
IL-7Rα (CD127), common gamma chain (γc or CD132)
Bioactivity
Measured by its ability to induce PHA-activated human PBL proliferation
Cell Type
B cells, Hematopoietic stem and progenitors
Biology Area
Immunology, Stem Cells
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Park LS, et al. 1990. J Exp Med. 171:1073-89.
  2. Fry TJ & Mackall CL. 2002. Blood. 99:3892-904.
  3. Lai L, et al. 2006. Blood. 107:1776-84.
  4. Link A, et al. 2007. Nat Immunol. 8:1255-65.
  5. Wofford JA, et al. 2008. Blood. 111:2101-11.
  6. Parrish YK, et al. 2009. J Immunol. 182:4255-66.
  7. Saini M, et al. 2009. Blood. 113:5793-800.
  8. Guimond M, et al. 2009. Nat Immunol. 10:149-57.
Gene ID
3574 View all products for this Gene ID
UniProt
View information about IL-7 on UniProt.org

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 1    Revision Date: 05.09.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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